Sensitive Detection of Minimal Residual Disease in Patients Treated for Early-Stage Breast Cancer

被引：136

作者：

Parsons, Heather A. ^{[1
]}

Rhoades, Justin ^{[2
]}

Reed, Sarah C. ^{[2
]}

Gydush, Gregory ^{[2
]}

Ram, Priyanka ^{[1
]}

Exman, Pedro ^{[1
]}

Xiong, Kan ^{[2
]}

Lo, Christopher C. ^{[2
,3
]}

Li, Tianyu ^{[4
]}

Fleharty, Mark ^{[2
]}

Kirkner, Gregory J. ^{[1
]}

Rotem, Denisse ^{[2
]}

Cohen, Ofir ^{[1
,2
]}

Yu, Fangyan ^{[5
,6
]}

Fitarelli-Kiehl, Mariana ^{[5
,6
]}

Leong, Ka Wai ^{[5
,6
]}

Hughes, Melissa E. ^{[1
]}

Rosenberg, Shoshana M. ^{[1
]}

Collins, Laura C. ^{[7
,8
]}

Miller, Kathy D. ^{[9
]}

Blumenstiel, Brendan ^{[2
]}

Trippa, Lorenzo ^{[4
]}

Cibulskis, Carrie ^{[2
]}

Neuberg, Donna S. ^{[4
]}

DeFelice, Matthew ^{[2
]}

Freeman, Samuel S. ^{[2
]}

Lennon, Niall J. ^{[2
]}

Wagle, Nikhil ^{[1
]}

Ha, Gavin ^{[10
]}

Stover, Daniel G. ^{[11
]}

Choudhury, Atish D. ^{[1
]}

Getz, Gad ^{[2
]}

Winer, Eric P. ^{[1
]}

Meyerson, Matthew ^{[1
,2
]}

Lin, Nancy U. ^{[1
]}

Krop, Ian ^{[1
]}

Love, J. Christopher ^{[2
,12
]}

Makrigiorgos, G. Mike ^{[2
]}

Partridge, Ann H. ^{[1
]}

Mayer, Erica L. ^{[1
]}

Golub, Todd R. ^{[2
,13
,14
]}

Adalsteinsson, Viktor A. ^{[2
,12
]}

机构：

[1] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA

[2] Broad Inst MIT & Harvard, Cambridge, MA USA

[3] Boston Univ, Sch Publ Hlth, Boston, MA 02215 USA

[4] Dana Farber Canc Inst, Div Biostat, Dept Data Sci, Boston, MA 02115 USA

[5] Dana Farber Canc Inst, Dept Radiat Oncol, Boston, MA 02115 USA

[6] Harvard Med Sch, Brigham & Womens Hosp, Boston, MA 02115 USA

[7] Beth Israel Deaconess Med Ctr, Dept Pathol, 330 Brookline Ave, Boston, MA 02215 USA

[8] Harvard Med Sch, Boston, MA 02115 USA

[9] Indiana Univ, Melvin & Bren Simon Canc Ctr, Indianapolis, IN 46204 USA

[10] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Serv, 1124 Columbia St, Seattle, WA 98104 USA

[11] Ohio State Univ, Comprehens Canc Ctr, Med Oncol, Columbus, OH 43210 USA

[12] MIT, Koch Inst Integrat Canc Res, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[13] Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02115 USA

[14] Howard Hughes Med Inst, Chevy Chase, MD USA

来源：

CLINICAL CANCER RESEARCH | 2020年 / 26卷 / 11期

关键词：

LUNG-CANCER; THERAPY;

D O I：

10.1158/1078-0432.CCR-19-3005

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Purpose: Existing cell-free DNA (cfDNA) methods lack the sensitivity needed for detecting minimal residual disease (MRD) following therapy. We developed a test for tracking hundreds of patient-specific mutations to detect MRD with a 1,000-fold lower error rate than conventional sequencing. Experimental Design: We compared the sensitivity of our approach to digital droplet PCR (ddPCR) in a dilution series, then retrospectively identified two cohorts of patients who had undergone prospective plasma sampling and clinical data collection: 16 patients with ER+/HER2- metastatic breast cancer (MBC) sampled within 6 months following metastatic diagnosis and 142 patients with stage 0 to III breast cancer who received curative-intent treatment with most sampled at surgery and 1 year postoperative. We performed whole-exome sequencing of tumors and designed individualized MRD tests, which we applied to serial cfDNA samples. Results: Our approach was 100-fold more sensitive than ddPCR when tracking 488 mutations, but most patients had fewer identifiable tumor mutations to track in cfDNA (median = 57; range = 2-346). Clinical sensitivity was 81% (n = 13/16) in newly diagnosed MBC, 23% (n = 7/30) at postoperative and 19% (n = 6/32) at 1 year in early-stage disease, and highest in patients with the most tumor mutations available to track. MRD detection at 1 year was strongly associated with distant recurrence [HR = 20.8; 95% confidence interval, 7.3-58.9]. Median lead time from first positive sample to recurrence was 18.9 months (range = 3.4-39.2 months). Conclusions: Tracking large numbers of individualized tumor mutations in cfDNA can improve MRD detection, but its sensitivity is driven by the number of tumor mutations available to track.

引用

页码：2556 / 2564

页数：9

共 22 条

[1] Phylogenetic ctDNA analysis depicts early-stage lung cancer evolution [J].

Abbosh, Christopher ;

Birkbak, Nicolai J. ;

Wilson, Gareth A. ;

Jamal-Hanjani, Mariam ;

Constantin, Tudor ;

Salari, Raheleh ;

Le Quesne, John ;

Moore, David A. ;

Veeriah, Selvaraju ;

Rosenthal, Rachel ;

Marafioti, Teresa ;

Kirkizlar, Eser ;

Watkins, Thomas B. K. ;

McGranahan, Nicholas ;

Ward, Sophia ;

Martinson, Luke ;

Riley, Joan ;

Fraioli, Francesco ;

Al Bakir, Maise ;

Gronroos, Eva ;

Zambrana, Francisco ;

Endozo, Raymondo ;

Bi, Wenya Linda ;

Fennessy, Fiona M. ;

Sponer, Nicole ;

Johnson, Diana ;

Laycock, Joanne ;

Shafi, Seema ;

Czyzewska-Khan, Justyna ;

Rowan, Andrew ;

Chambers, Tim ;

Matthews, Nik ;

Turajlic, Samra ;

Hiley, Crispin ;

Lee, Siow Ming ;

Forster, Martin D. ;

Ahmad, Tanya ;

Falzon, Mary ;

Borg, Elaine ;

Lawrence, David ;

Hayward, Martin ;

Kolvekar, Shyam ;

Panagiotopoulos, Nikolaos ;

Janes, Sam M. ;

Thakrar, Ricky ;

Ahmed, Asia ;

Blackhall, Fiona ;

Summers, Yvonne ;

Hafez, Dina ;

Naik, Ashwini .

NATURE, 2017, 545 (7655) :446-+

[2] Scalable whole-exome sequencing of cell-free DNA reveals high concordance with metastatic tumors [J].

Adalsteinsson, Viktor A. ;

Ha, Gavin ;

Freeman, Samuel S. ;

Choudhury, Atish D. ;

Stover, Daniel G. ;

Parsons, Heather A. ;

Gydush, Gregory ;

Reed, Sarah C. ;

Rotem, Denisse ;

Rhoades, Justin ;

Loginov, Denis ;

Livitz, Dimitri ;

Rosebrock, Daniel ;

Leshchiner, Ignaty ;

Kim, Jaegil ;

Stewart, Chip ;

Rosenberg, Mara ;

Francis, Joshua M. ;

Zhang, Cheng-Zhong ;

Cohen, Ofir ;

Oh, Coyin ;

Ding, Huiming ;

Polak, Paz ;

Lloyd, Max ;

Mahmud, Sairah ;

Helvie, Karla ;

Merrill, Margaret S. ;

Santiago, Rebecca A. ;

O'Connor, Edward P. ;

Jeong, Seong H. ;

Leeson, Rachel ;

Barry, Rachel M. ;

Kramkowski, Joseph F. ;

Zhang, Zhenwei ;

Polacek, Laura ;

Lohr, Jens G. ;

Schleicher, Molly ;

Lipscomb, Emily ;

Saltzman, Andrea ;

Oliver, Nelly M. ;

Marini, Lori ;

Waks, Adrienne G. ;

Harshman, Lauren C. ;

Tolaney, Sara M. ;

Van Allen, Eliezer M. ;

Winer, Eric P. ;

Lin, Nancy U. ;

Nakabayashi, Mari ;

Taplin, Mary-Ellen ;

Johannessen, Cory M. .

NATURE COMMUNICATIONS, 2017, 8

[3] Comparisons between different polychemotherapy regimens for early breast cancer: meta-analyses of long-term outcome among 100 000 women in 123 randomised trials [J].

Albain, K. ;

Anderson, S. ;

Arriagada, R. ;

Barlow, W. ;

Bergh, J. ;

Bliss, J. ;

Buyse, M. ;

Cameron, D. ;

Carrasco, E. ;

Clarke, M. ;

Correa, C. ;

Coates, A. ;

Collins, R. ;

Costantino, J. ;

Cutter, D. ;

Cuzick, J. ;

Darby, S. ;

Davidson, N. ;

Davies, C. ;

Davies, K. ;

Delmestri, A. ;

Di Leo, A. ;

Dowsett, M. ;

Elphinstone, P. ;

Evans, V. ;

Ewertz, M. ;

Gelber, R. ;

Gettins, L. ;

Geyer, C. ;

Goldhirsch, A. ;

Godwin, J. ;

Gray, R. ;

Gregory, C. ;

Hayes, D. ;

Hill, C. ;

Ingle, J. ;

Jakesz, R. ;

James, S. ;

Kaufmann, M. ;

Kerr, A. ;

MacKinnon, E. ;

McGale, P. ;

McHugh, T. ;

Norton, L. ;

Ohashi, Y. ;

Paik, S. ;

Pan, H. C. ;

Perez, E. ;

Peto, R. ;

Piccart, M. .

LANCET, 2012, 379 (9814) :432-444

[4] Adjuvant Endocrine Therapy for Women With Hormone Receptor-Positive Breast Cancer: ASCO Clinical Practice Guideline Focused Update [J].

Burstein, Harold J. ;

Lacchetti, Christina ;

Anderson, Holly ;

Buchholz, Thomas A. ;

Davidson, Nancy E. ;

Gelmon, Karen A. ;

Giordano, Sharon H. ;

Hudis, Clifford A. ;

Solky, Alexander J. ;

Stearns, Vered ;

Winer, Eric P. ;

Griggs, Jennifer J. .

JOURNAL OF CLINICAL ONCOLOGY, 2019, 37 (05) :423-+

[5] Early Detection of Molecular Residual Disease in Localized Lung Cancer by Circulating Tumor DNA Profiling [J].

Chaudhuri, Aadel A. ;

Chabon, Jacob J. ;

Lovejoy, Alexander F. ;

Newman, Aaron M. ;

Stehr, Henning ;

Azad, Tej D. ;

Khodadoust, Michael S. ;

Esfahani, Mohammad Shahrokh ;

Liu, Chih Long ;

Zhou, Li ;

Scherer, Florian ;

Kurtz, David M. ;

Say, Carmen ;

Carter, Justin N. ;

Merriott, David J. ;

Dudley, Jonathan C. ;

Binkley, Michael S. ;

Modlin, Leslie ;

Padda, Sukhmani K. ;

Gensheimer, Michael F. ;

West, Robert B. ;

Shrager, Joseph B. ;

Neal, Joel W. ;

Wakelee, Heather A. ;

Loo, Billy W., Jr. ;

Alizadeh, Ash A. ;

Diehn, Maximilian .

CANCER DISCOVERY, 2017, 7 (12) :1394-1403

[6] Tumor fraction in cell-free DNA as a biomarker in prostate cancer [J].

Choudhury, Atish D. ;

Werner, Lillian ;

Francini, Edoardo ;

Wei, Xiao X. ;

Ha, Gavin ;

Freeman, Samuel S. ;

Rhoades, Justin ;

Reed, Sarah C. ;

Gydush, Gregory ;

Rotem, Denisse ;

Lo, Christopher ;

Taplin, Mary-Ellen ;

Harshman, Lauren C. ;

Zhang, Zhenwei ;

O'Connor, Edward P. ;

Stover, Daniel G. ;

Parsons, Heather A. ;

Getz, Gad ;

Meyerson, Matthew ;

Love, J. Christopher ;

Hahn, William C. ;

Adalsteinsson, Viktor A. .

JCI INSIGHT, 2018, 3 (21)

[7] Early Detection of Metastatic Relapse and Monitoring of Therapeutic Efficacy by Ultra-Deep Sequencing of Plasma Cell-Free DNA in Patients With Urothelial Bladder Carcinoma [J].

Christensen, Emil ;

Birkenkamp-Demtroder, Karin ;

Sethi, Himanshu ;

Shchegrova, Svetlana ;

Salari, Raheleh ;

Nordentoft, Iver ;

Wu, Hsin-Ta ;

Knudsen, Michael ;

Lamy, Philippe ;

Lindskrog, Sia Viborg ;

Taber, Ann ;

Balcioglu, Mustafa ;

Vang, Soren ;

Assaf, Zoe ;

Sharma, Shruti ;

Tin, Antony S. ;

Srinivasan, Ramya ;

Hafez, Dina ;

Reinert, Thomas ;

Navarro, Samantha ;

Olson, Alexander ;

Ram, Rosalyn ;

Dashner, Scott ;

Rabinowitz, Matthew ;

Billings, Paul ;

Sigurjonsson, Styrmir ;

Andersen, Claus Lindbjerg ;

Swenerton, Ryan ;

Aleshin, Alexey ;

Zimmermann, Bernhard ;

Agerbaek, Mads ;

Lin, Cheng-Ho Jimmy ;

Jensen, JOrgen Bjerggaard ;

Dyrskjot, Lars .

JOURNAL OF CLINICAL ONCOLOGY, 2019, 37 (18) :1547-1557

[8] Personalized Detection of Circulating Tumor DNA Antedates Breast Cancer Metastatic Recurrence [J].

Coombes, Raoul Charles ;

Page, Karen ;

Salari, Raheleh ;

Hastings, Robert K. ;

Armstrong, Anne ;

Ahmed, Samreen ;

Ali, Simak ;

Cleator, Susan ;

Kenny, Laura ;

Stebbing, Justin ;

Rutherford, Mark ;

Sethi, Himanshu ;

Boydell, Anna ;

Swenerton, Ryan ;

Fernandez-Garcia, Daniel ;

Gleason, Kelly L. T. ;

Goddard, Katie ;

Guttery, David S. ;

Assaf, Zoe J. ;

Wu, Hsin-Ta ;

Natarajan, Prashanthi ;

Moore, David A. ;

Primrose, Lindsay ;

Dashner, Scott ;

Tin, Antony S. ;

Balcioglu, Mustafa ;

Srinivasan, Ramya ;

Shchegrova, Svetlana V. ;

Olson, Alexander ;

Hafez, Dina ;

Billings, Paul ;

Aleshin, Alexey ;

Rehman, Farah ;

Toghill, Bradley J. ;

Hills, Allison ;

Louie, Maggie C. ;

Lin, Cheng-Ho Jimmy ;

Zimmermann, Bernhard G. ;

Shaw, Jaqueline A. .

CLINICAL CANCER RESEARCH, 2019, 25 (14) :4255-4263

[9] Aromatase inhibitors versus tamoxifen in early breast cancer: patient-level meta-analysis of the randomised trials [J].

Dowsett, M. ;

Forbes, J. F. ;

Bradley, R. ;

Ingle, J. ;

Aihara, T. ;

Bliss, J. ;

Boccardo, F. ;

Coates, A. ;

Coombes, R. C. ;

Cuzick, J. ;

Dubsky, P. ;

Gnant, M. ;

Kaufmann, M. ;

Kilburn, L. ;

Perrone, F. ;

Rea, D. ;

Thuerlimann, B. ;

van de Velde, C. ;

Pan, H. ;

Peto, R. ;

Davies, C. ;

Gray, R. ;

Baum, M. ;

Buzdar, A. ;

Sestak, I. ;

Markopoulos, C. ;

Fesl, C. ;

Jakesz, R. ;

Colleoni, M. ;

Gelber, R. ;

Regan, M. ;

von Minckwitz, G. ;

Snowdon, C. ;

Goss, P. ;

Pritchard, K. ;

Anderson, S. ;

Costantino, J. ;

Mamounas, E. ;

Ohashi, Y. ;

Watanabe, T. ;

Bastiaannet, E. .

LANCET, 2015, 386 (10001) :1341-1352

[10] Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods [J].

Ferlay, J. ;

Colombet, M. ;

Soerjomataram, I. ;

Mathers, C. ;

Parkin, D. M. ;

Pineros, M. ;

Znaor, A. ;

Bray, F. .

INTERNATIONAL JOURNAL OF CANCER, 2019, 144 (08) :1941-1953

← 1 2 3 →

共 22 条

[1] Phylogenetic ctDNA analysis depicts early-stage lung cancer evolution [J].

Abbosh, Christopher ;

Birkbak, Nicolai J. ;

Wilson, Gareth A. ;

Jamal-Hanjani, Mariam ;

Constantin, Tudor ;

Salari, Raheleh ;

Le Quesne, John ;

Moore, David A. ;

Veeriah, Selvaraju ;

Rosenthal, Rachel ;

Marafioti, Teresa ;

Kirkizlar, Eser ;

Watkins, Thomas B. K. ;

McGranahan, Nicholas ;

Ward, Sophia ;

Martinson, Luke ;

Riley, Joan ;

Fraioli, Francesco ;

Al Bakir, Maise ;

Gronroos, Eva ;

Zambrana, Francisco ;

Endozo, Raymondo ;

Bi, Wenya Linda ;

Fennessy, Fiona M. ;

Sponer, Nicole ;

Johnson, Diana ;

Laycock, Joanne ;

Shafi, Seema ;

Czyzewska-Khan, Justyna ;

Rowan, Andrew ;

Chambers, Tim ;

Matthews, Nik ;

Turajlic, Samra ;

Hiley, Crispin ;

Lee, Siow Ming ;

Forster, Martin D. ;

Ahmad, Tanya ;

Falzon, Mary ;

Borg, Elaine ;

Lawrence, David ;

Hayward, Martin ;

Kolvekar, Shyam ;

Panagiotopoulos, Nikolaos ;

Janes, Sam M. ;

Thakrar, Ricky ;

Ahmed, Asia ;

Blackhall, Fiona ;

Summers, Yvonne ;

Hafez, Dina ;

Naik, Ashwini .

NATURE, 2017, 545 (7655) :446-+

[2] Scalable whole-exome sequencing of cell-free DNA reveals high concordance with metastatic tumors [J].

Adalsteinsson, Viktor A. ;

Ha, Gavin ;

Freeman, Samuel S. ;

Choudhury, Atish D. ;

Stover, Daniel G. ;

Parsons, Heather A. ;

Gydush, Gregory ;

Reed, Sarah C. ;

Rotem, Denisse ;

Rhoades, Justin ;

Loginov, Denis ;

Livitz, Dimitri ;

Rosebrock, Daniel ;

Leshchiner, Ignaty ;

Kim, Jaegil ;

Stewart, Chip ;

Rosenberg, Mara ;

Francis, Joshua M. ;

Zhang, Cheng-Zhong ;

Cohen, Ofir ;

Oh, Coyin ;

Ding, Huiming ;

Polak, Paz ;

Lloyd, Max ;

Mahmud, Sairah ;

Helvie, Karla ;

Merrill, Margaret S. ;

Santiago, Rebecca A. ;

O'Connor, Edward P. ;

Jeong, Seong H. ;

Leeson, Rachel ;

Barry, Rachel M. ;

Kramkowski, Joseph F. ;

Zhang, Zhenwei ;

Polacek, Laura ;

Lohr, Jens G. ;

Schleicher, Molly ;

Lipscomb, Emily ;

Saltzman, Andrea ;

Oliver, Nelly M. ;

Marini, Lori ;

Waks, Adrienne G. ;

Harshman, Lauren C. ;

Tolaney, Sara M. ;

Van Allen, Eliezer M. ;

Winer, Eric P. ;

Lin, Nancy U. ;

Nakabayashi, Mari ;

Taplin, Mary-Ellen ;

Johannessen, Cory M. .

NATURE COMMUNICATIONS, 2017, 8

[3] Comparisons between different polychemotherapy regimens for early breast cancer: meta-analyses of long-term outcome among 100 000 women in 123 randomised trials [J].

Albain, K. ;

Anderson, S. ;

Arriagada, R. ;

Barlow, W. ;

Bergh, J. ;

Bliss, J. ;

Buyse, M. ;

Cameron, D. ;

Carrasco, E. ;

Clarke, M. ;

Correa, C. ;

Coates, A. ;

Collins, R. ;

Costantino, J. ;

Cutter, D. ;

Cuzick, J. ;

Darby, S. ;

Davidson, N. ;

Davies, C. ;

Davies, K. ;

Delmestri, A. ;

Di Leo, A. ;

Dowsett, M. ;

Elphinstone, P. ;

Evans, V. ;

Ewertz, M. ;

Gelber, R. ;

Gettins, L. ;

Geyer, C. ;

Goldhirsch, A. ;

Godwin, J. ;

Gray, R. ;

Gregory, C. ;

Hayes, D. ;

Hill, C. ;

Ingle, J. ;

Jakesz, R. ;

James, S. ;

Kaufmann, M. ;

Kerr, A. ;

MacKinnon, E. ;

McGale, P. ;

McHugh, T. ;

Norton, L. ;

Ohashi, Y. ;

Paik, S. ;

Pan, H. C. ;

Perez, E. ;

Peto, R. ;

Piccart, M. .

LANCET, 2012, 379 (9814) :432-444

[4] Adjuvant Endocrine Therapy for Women With Hormone Receptor-Positive Breast Cancer: ASCO Clinical Practice Guideline Focused Update [J].

Burstein, Harold J. ;

Lacchetti, Christina ;

Anderson, Holly ;

Buchholz, Thomas A. ;

Davidson, Nancy E. ;

Gelmon, Karen A. ;

Giordano, Sharon H. ;

Hudis, Clifford A. ;

Solky, Alexander J. ;

Stearns, Vered ;

Winer, Eric P. ;

Griggs, Jennifer J. .

JOURNAL OF CLINICAL ONCOLOGY, 2019, 37 (05) :423-+

[5] Early Detection of Molecular Residual Disease in Localized Lung Cancer by Circulating Tumor DNA Profiling [J].

Chaudhuri, Aadel A. ;

Chabon, Jacob J. ;

Lovejoy, Alexander F. ;

Newman, Aaron M. ;

Stehr, Henning ;

Azad, Tej D. ;

Khodadoust, Michael S. ;

Esfahani, Mohammad Shahrokh ;

Liu, Chih Long ;

Zhou, Li ;

Scherer, Florian ;

Kurtz, David M. ;

Say, Carmen ;

Carter, Justin N. ;

Merriott, David J. ;

Dudley, Jonathan C. ;

Binkley, Michael S. ;

Modlin, Leslie ;

Padda, Sukhmani K. ;

Gensheimer, Michael F. ;

West, Robert B. ;

Shrager, Joseph B. ;

Neal, Joel W. ;

Wakelee, Heather A. ;

Loo, Billy W., Jr. ;

Alizadeh, Ash A. ;

Diehn, Maximilian .

CANCER DISCOVERY, 2017, 7 (12) :1394-1403

[6] Tumor fraction in cell-free DNA as a biomarker in prostate cancer [J].

Choudhury, Atish D. ;

Werner, Lillian ;

Francini, Edoardo ;

Wei, Xiao X. ;

Ha, Gavin ;

Freeman, Samuel S. ;

Rhoades, Justin ;

Reed, Sarah C. ;

Gydush, Gregory ;

Rotem, Denisse ;

Lo, Christopher ;

Taplin, Mary-Ellen ;

Harshman, Lauren C. ;

Zhang, Zhenwei ;

O'Connor, Edward P. ;

Stover, Daniel G. ;

Parsons, Heather A. ;

Getz, Gad ;

Meyerson, Matthew ;

Love, J. Christopher ;

Hahn, William C. ;

Adalsteinsson, Viktor A. .

JCI INSIGHT, 2018, 3 (21)

[7] Early Detection of Metastatic Relapse and Monitoring of Therapeutic Efficacy by Ultra-Deep Sequencing of Plasma Cell-Free DNA in Patients With Urothelial Bladder Carcinoma [J].

Christensen, Emil ;

Birkenkamp-Demtroder, Karin ;

Sethi, Himanshu ;

Shchegrova, Svetlana ;

Salari, Raheleh ;

Nordentoft, Iver ;

Wu, Hsin-Ta ;

Knudsen, Michael ;

Lamy, Philippe ;

Lindskrog, Sia Viborg ;

Taber, Ann ;

Balcioglu, Mustafa ;

Vang, Soren ;

Assaf, Zoe ;

Sharma, Shruti ;

Tin, Antony S. ;

Srinivasan, Ramya ;

Hafez, Dina ;

Reinert, Thomas ;

Navarro, Samantha ;

Olson, Alexander ;

Ram, Rosalyn ;

Dashner, Scott ;

Rabinowitz, Matthew ;

Billings, Paul ;

Sigurjonsson, Styrmir ;

Andersen, Claus Lindbjerg ;

Swenerton, Ryan ;

Aleshin, Alexey ;

Zimmermann, Bernhard ;

Agerbaek, Mads ;

Lin, Cheng-Ho Jimmy ;

Jensen, JOrgen Bjerggaard ;

Dyrskjot, Lars .

JOURNAL OF CLINICAL ONCOLOGY, 2019, 37 (18) :1547-1557

[8] Personalized Detection of Circulating Tumor DNA Antedates Breast Cancer Metastatic Recurrence [J].

Coombes, Raoul Charles ;

Page, Karen ;

Salari, Raheleh ;

Hastings, Robert K. ;

Armstrong, Anne ;

Ahmed, Samreen ;

Ali, Simak ;

Cleator, Susan ;

Kenny, Laura ;

Stebbing, Justin ;

Rutherford, Mark ;

Sethi, Himanshu ;

Boydell, Anna ;

Swenerton, Ryan ;

Fernandez-Garcia, Daniel ;

Gleason, Kelly L. T. ;

Goddard, Katie ;

Guttery, David S. ;

Assaf, Zoe J. ;

Wu, Hsin-Ta ;

Natarajan, Prashanthi ;

Moore, David A. ;

Primrose, Lindsay ;

Dashner, Scott ;

Tin, Antony S. ;

Balcioglu, Mustafa ;

Srinivasan, Ramya ;

Shchegrova, Svetlana V. ;

Olson, Alexander ;

Hafez, Dina ;

Billings, Paul ;

Aleshin, Alexey ;

Rehman, Farah ;

Toghill, Bradley J. ;

Hills, Allison ;

Louie, Maggie C. ;

Lin, Cheng-Ho Jimmy ;

Zimmermann, Bernhard G. ;

Shaw, Jaqueline A. .

CLINICAL CANCER RESEARCH, 2019, 25 (14) :4255-4263

[9] Aromatase inhibitors versus tamoxifen in early breast cancer: patient-level meta-analysis of the randomised trials [J].

Dowsett, M. ;

Forbes, J. F. ;

Bradley, R. ;

Ingle, J. ;

Aihara, T. ;

Bliss, J. ;

Boccardo, F. ;

Coates, A. ;

Coombes, R. C. ;

Cuzick, J. ;

Dubsky, P. ;

Gnant, M. ;

Kaufmann, M. ;

Kilburn, L. ;

Perrone, F. ;

Rea, D. ;

Thuerlimann, B. ;

van de Velde, C. ;

Pan, H. ;

Peto, R. ;

Davies, C. ;

Gray, R. ;

Baum, M. ;

Buzdar, A. ;

Sestak, I. ;

Markopoulos, C. ;

Fesl, C. ;

Jakesz, R. ;

Colleoni, M. ;

Gelber, R. ;

Regan, M. ;

von Minckwitz, G. ;

Snowdon, C. ;

Goss, P. ;

Pritchard, K. ;

Anderson, S. ;

Costantino, J. ;

Mamounas, E. ;

Ohashi, Y. ;

Watanabe, T. ;

Bastiaannet, E. .

LANCET, 2015, 386 (10001) :1341-1352

[10] Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods [J].

Ferlay, J. ;

Colombet, M. ;

Soerjomataram, I. ;

Mathers, C. ;

Parkin, D. M. ;

Pineros, M. ;

Znaor, A. ;

Bray, F. .

INTERNATIONAL JOURNAL OF CANCER, 2019, 144 (08) :1941-1953

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