High-Throughput Fluorescence-Activated Nanoscale Subcellular Sorter with Single-Molecule Sensitivity

被引：7
作者：
Schiro, Perry G. [1 ]
Gadd, Jennifer C. [1 ]
Yen, Gloria S. [1 ]
Chiu, Daniel T. [1 ]
机构：
[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA
来源：
JOURNAL OF PHYSICAL CHEMISTRY B | 2012年 / 116卷 / 35期
基金：
美国国家卫生研究院;
关键词：
7S SNARE COMPLEXES; FLOW-CYTOMETRY; MICROFLUIDIC DEVICES; CELLS; ACCUMULATION; MANIPULATION; EXPRESSION; DROPLETS; SYSTEM; IV;
D O I：
10.1021/jp3019233
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Recent single-cell and single-molecule studies have shown that a variety of subpopulations exist within biological systems, such as synaptic vesicles, that have previously been overlooked in common bulk studies. By isolating and enriching these various subpopulations, detailed analysis with a variety of analytical techniques can be done to further understand the role that various subpopulations play in cellular dynamics and how alterations to these subpopulations affect the overall function of the biological system. Previous sorters lack the sensitivity, sorting speed, and efficiency to isolate synaptic vesicles and other nanoscale systems. This paper describes the development of a fluorescence-activated nanoscale subcellular sorter that can sort nearly 10 million objects per hour with single-molecule sensitivity. Utilizing a near-nanoscale channel system, we were able to achieve upward of 91% recovery of desired objects with a 99.7% purity.
引用
收藏
页码：10490 / 10495
页数：6
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