Agrobacterium-mediated transformation of apricot (Prunus armeniaca L.) leaf explants

被引：45

作者：

Petri, Cesar ^{[1
]}

Wang, Hong ^{[1
]}

Alburquerque, Nuria ^{[1
]}

Faize, Mohamed ^{[1
]}

Burgos, Lorenzo ^{[1
]}

机构：

[1] CEBAS CSIC, Murcia 30100, Spain

来源：

PLANT CELL REPORTS | 2008年 / 27卷 / 08期

关键词：

apricot; sgfp; uidA; Prunus; transformation;

D O I：

10.1007/s00299-008-0550-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A protocol for Agrobacterium-mediated stable transformation for scored, whole leaf explants of the apricot (Prunus armeniaca) cultivar Helena was developed. Regenerated shoots were selected using a two-step increased concentrations of paromomycin sulphate. Different factors affecting survival of transformed buds, including possible toxicity of green fluorescent protein (GFP) and time of exposure to high cytokine concentration in the regeneration medium, were examined. Transformation efficiency, based on PCR analysis of individual putative transformed shoots from independent lines was 5.6%, when optimal conditions for bud survival were provided. Southern blot analysis on four randomly chosen PCR-positive shoots confirmed the presence of the nptII transgene. This is the first time that stable transformation of an apricot cultivar is reported and constitutes also one of the few reports on the transformation of Prunus cultivars.

引用

页码：1317 / 1324

页数：8

共 43 条

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