Comparison of Asymptomatic Bacteriuria Escherichia coli Isolates from Healthy Individuals versus Those from Hospital Patients Shows that Long-Term Bladder Colonization Selects for Attenuated Virulence Phenotypes

被引:53
作者
Salvador, Ellaine [1 ]
Wagenlehner, Florian [2 ]
Koehler, Christian-Daniel [1 ,3 ]
Mellmann, Alexander [3 ]
Hacker, Joerg [4 ]
Svanborg, Catharina [5 ]
Dobrindt, Ulrich [1 ,3 ]
机构
[1] Univ Wurzburg, Inst Mol Biol Infect Dis, Wurzburg, Germany
[2] Univ Giessen, Clin Urol Pediat Urol & Androl, Giessen, Germany
[3] Univ Munster, Inst Hyg, Munster, Germany
[4] German Acad Sci Leopoldina, Halle, Germany
[5] Lund Univ, Inst Lab Med, Dept Microbiol Immunol & Glycobiol, Lund, Sweden
基金
英国医学研究理事会;
关键词
URINARY-TRACT-INFECTIONS; PSEUDOMONAS-AERUGINOSA; INNATE IMMUNITY; STRAINS; SUSCEPTIBILITY; INFERENCE; STRAIN-83972; PERSISTENCE; GENETICS; CHILDREN;
D O I
10.1128/IAI.06191-11
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Asymptomatic bacteriuria (ABU) is a condition where bacteria stably colonize the urinary tract, in a manner closely resembling commensalism at other mucosa! sites. The patients carry >10(5) CFU/ml for extended periods of time and rarely develop symptoms. Contrasting the properties of ABU strains to those of uropathogenic isolates causing symptomatic infection is therefore highly relevant to understand mechanisms of bacterial adaptation. The prototype ABU strain Escherichia coli 83972 has a smaller genome than uropathogenic E. coli (UPEC) strains with deletions or point mutations in several virulence genes, suggesting that ABU strains undergo a programmed reductive evolution within human hosts. This study addressed if these observations can be generalized. Strains causing ABU in outpatients or hospitalized patients after catheterization or other invasive procedures were compared to commensal E. coli isolates from the intestinal flora of healthy individuals. Notably, clonal complex 73 (CC73) was a prominent phylogenetic lineage dominated by ABU isolates. ABU isolates from outpatients and hospitalized patients had a similar overall virulence gene repertoire, which distinguished them from many commensals, but typical UPEC virulence genes were less frequently attenuated in hospital strains than in outpatient strains or commensals. The decreased virulence potential of outpatient ABU isolates relative to that of ABU strains from hospitalized patients supports the hypothesis that loss of expression or decay of virulence genes facilitates long-term carriage and adaptation to host environments.
引用
收藏
页码:668 / 678
页数:11
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