The periodontopathic bacteria in placenta, saliva and subgingival plaque of threatened preterm labor and preterm low birth weight cases: a longitudinal study in Japanese pregnant women

被引:30
作者
Ye, Changchang [1 ,2 ,3 ]
Katagiri, Sayaka [4 ]
Miyasaka, Naoyuki [5 ]
Kobayashi, Hiroaki [4 ]
Khemwong, Thatawee [4 ]
Nagasawa, Toshiyuki [6 ]
Izumi, Yuichi [4 ]
机构
[1] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu, Peoples R China
[2] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Chengdu, Peoples R China
[3] Sichuan Univ, West China Hosp Stomatol, Dept Periodontol, Chengdu, Peoples R China
[4] Tokyo Med & Dent Univ, Grad Sch Med & Dent Sci, Dept Periodontol, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138549, Japan
[5] Tokyo Med & Dent Univ, Dept Pediat Perinatal & Maternal Med, Med Hosp, Tokyo, Japan
[6] Hlth Sci Univ Hokkaido, Sch Dent, Dept Oral Rehabil, Div Periodontol & Endodontol, Sapporo, Hokkaido, Japan
基金
日本学术振兴会; 中国国家自然科学基金;
关键词
Periodontal pathogens; Anti-periodontopathic immunoglobulin G; Threatened preterm labor; Preterm low birth weight; PORPHYROMONAS-GINGIVALIS; FUSOBACTERIUM-NUCLEATUM; POLYMICROBIAL SYNERGY; ORAL BACTERIA; OUTCOMES; RISK; DYSBIOSIS; INFECTION; PREMATURE; DISEASE;
D O I
10.1007/s00784-020-03287-4
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives This study determined the quantity of periodontopathic bacteria in saliva, subgingival plaque, and placenta on the threatened preterm labor (TPL) and preterm low birth weight (PLBW) subjects in order to identify specific periodontal pathogens with high association to adverse pregnancy outcomes. Methods We used real-time PCR with TaqMan probe and ELISA to detect the amount of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, and Prevotella intermedia in subgingival plaque, saliva, and placenta tissue, in addition to serum IgG titers against these bacteria in 28 patients with TPL and 36 healthy pregnant women. Results Thirteen of 64 births delivered PLBW infants. All 6 periodontopathic bacteria were detected in the placenta samples. The amount of F. nucleatum and detection frequency of T. denticola in placental samples was significantly higher in the TPL group than in the healthy group. Meanwhile, the age, anti-P. gingival IgG in serum, amount of P. gingivalis and T. forsythia in plaque samples, detection frequency of P. intermedia in saliva, and percentage of pocket probing depth >= 5 mm were higher in TPL-PLBW births than those in TPL-Healthy delivery (HD) group and/or in H-HD group. Ordinal logistic regression analysis revealed that the presence of F. nucleatum in placental tissues was significantly associated with TPL, while the maternal age was significantly associated with PLBW in TPL. Conclusion Our findings suggested all 6 bacteria may access the placenta. The increased presence of F. nucleatum in placenta might be related to TPL, while advanced maternal age might be associated with PLBW in TPL.
引用
收藏
页码:4261 / 4270
页数:10
相关论文
共 46 条
  • [1] Dental Infection of Porphyromonas gingivalis Induces Preterm Birth in Mice
    Ao, Min
    Miyauchi, Mutsumi
    Furusho, Hisako
    Inubushi, Toshihiro
    Kitagawa, Masae
    Nagasaki, Atsuhiro
    Sakamoto, Shinichi
    Kozai, Katsuyuki
    Takata, Takashi
    [J]. PLOS ONE, 2015, 10 (08):
  • [2] Oral bacteria in placental tissues: increased molecular detection in pregnant periodontitis patients
    Blanc, V.
    O'Valle, F.
    Pozo, E.
    Puertas, A.
    Leon, R.
    Mesa, F.
    [J]. ORAL DISEASES, 2015, 21 (07) : 905 - 912
  • [3] PERIODONTAL BACTERIA IN THE GENITAL TRACT: ARE THEY RELATED TO ADVERSE PREGNANCY OUTCOME?
    Cassini, M. A.
    Pilloni, A.
    Condo, S. G.
    Vitali, L. A.
    Pasquantonio, G.
    Cerroni, L.
    [J]. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 2013, 26 (04): : 931 - 939
  • [4] Champagne C M, 2000, J Int Acad Periodontol, V2, P9
  • [5] TRANSITORY BACTEREMIA
    COBE, HM
    [J]. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 1954, 7 (06): : 609 - 615
  • [6] Periodontal disease as a risk factor for adverse pregnancy outcomes: a systematic review and meta-analysis of case-control studies
    Corbella, Stefano
    Taschieri, Silvio
    Francetti, Luca
    De Siena, Francesca
    Del Fabbro, Massimo
    [J]. ODONTOLOGY, 2012, 100 (02) : 232 - 240
  • [7] Bacteremia due to periodontal probing: A clinical and microbiological investigation
    Daly, CG
    Mitchell, DH
    Highfield, JE
    Grossberg, DE
    Stewart, D
    [J]. JOURNAL OF PERIODONTOLOGY, 2001, 72 (02) : 210 - 214
  • [8] Term and preterm labour are associated with distinct microbial community structures in placental membranes which are independent of mode of delivery
    Doyle, R. M.
    Alber, D. G.
    Jones, H. E.
    Harris, K.
    Fitzgerald, F.
    Peebles, D.
    Klein, N.
    [J]. PLACENTA, 2014, 35 (12) : 1099 - 1101
  • [9] Maternal oral origin of Fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16S-23S rRNA gene intergenic transcribed spacer region
    Gonzales-Marin, Cecilia
    Spratt, David A.
    Allaker, Robert P.
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2013, 62 : 133 - 144
  • [10] Beyond the red complex and into more complexity: the polymicrobial synergy and dysbiosis (PSD) model of periodontal disease etiology
    Hajishengallis, G.
    Lamont, R. J.
    [J]. MOLECULAR ORAL MICROBIOLOGY, 2012, 27 (06) : 409 - 419