Large-scale, high-efficiency production of coffee somatic embryos

被引：7
作者：
de Rezende Maciel, Anna Lygia [1 ]
Rodrigues, Filipe Almendagna [2 ]
Pasqual, Moacir [2 ]
Siqueira de Carvalho, Carlos Henrique [3 ]
机构：
[1] Inst Fed Educ Ciencia & Tecnol Sul Minas Gerais, Campus Morro Preto, BR-37890000 Muzambinho, MG, Brazil
[2] Univ Fed Lavras, Dept Agr, Campus UFLA 3037, BR-37200000 Lavras, MG, Brazil
[3] Embrapa Cafe, Alameda Cafe 1000, BR-37026400 Varginha, MG, Brazil
来源：
CROP BREEDING AND APPLIED BIOTECHNOLOGY | 2016年 / 16卷 / 02期
关键词：
Vegetative propagation; biotechnology; tissue culture; Coffea arabica L; EMBRYOGENESIS; PLANTS; REGENERATION;
D O I：
10.1590/1984-70332016v16n2a16
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.
引用
收藏
页码：102 / 107
页数：6
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