Sugarcane straw and its phytochemicals as growth regulators of weed and crop plants

The present study investigates the effect of soil amended with sugarcane straw leachate and its constituents on root elongation of weed and crop plants. The influence of soil biotic and abiotic factors on plant growth were also evaluated through assays in both autoclaved soil and sand. In unsterile soil, straw leachates stimulated root growth of some test plants at 6 g dry straw ls(-1) and inhibited root elongation at higher concentrations. A bioassay guided process of the bioactive leachate constituents led to the isolation of vanillic, syringic and ferulic acids. These compounds were also assayed on the test plants in the three mentioned substrates. In unsterile soil, phenolics stimulated the growth of some species at 19 mg l(-1). Higher phenolics concentrations were inhibitory. The concentration needed to inhibit 25% root elongation (EC25) was calculated from the dose-response curves. The concentration of phenolics in the leachate (64 g dry straw l(-1)) was estimated to be 187 mg l(-1)(ferulic acid), 131 mg l(-1) (vanillic acid) and 20 mg l(-1) (Syringic acid). In general, these concentrations were below the EC25 values determined in unsterile soil indicating that these compounds cannot completely explain the strong inhibitory activity of sugarcane straw leachates. The role of soil factors on phytotoxicity of sugarcane straw leachate and its identified growth regulators is also discussed.