Comparison between conventional methods, ChromAgar Candida® and PCR method for the identification of Candida species in clinical isolates

被引:13
作者
Estrada-Barraza, Deyanira [1 ]
Davalos Martinez, Arturo [1 ]
Flores-Padilla, Luis [2 ]
Mendoza-De Elias, Roberto [1 ]
Octavio Sanchez-Vargas, Luis [1 ]
机构
[1] Univ Autonoma Ciudad Juarez, Inst Ciencias Biomed, Ciudad Juarez, Chihuahua, Mexico
[2] Hosp Reg Zona 6, Inst Mexicano Seguro Social, Dept Epidemiol, Ciudad Juarez, Chihuahua, Mexico
来源
REVISTA IBEROAMERICANA DE MICOLOGIA | 2011年 / 28卷 / 01期
关键词
Candida; Polymerase chain reaction; ATB ID32C; Identification; Clinical isolates; MEDICALLY IMPORTANT YEASTS; TOPOISOMERASE-II GENE; INTENSIVE-CARE-UNIT; RAPID IDENTIFICATION; PRESUMPTIVE IDENTIFICATION; MULTIPLEX PCR; API; 20C; DNA; ALBICANS; BLOOD;
D O I
10.1016/j.riam.2010.11.003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The increase in the incidence of yeast species causing fungemia in susceptible immunocompromised patients in the last two decades and the low sensitivity of conventional blood culture has led to the need to develop alternative approaches for the early detection and identification of causative species. The aim of this study was to compare the usefulness of molecular testing by the polymerase chain reaction (PCR) and conventional methods to identify clinical isolates of different species, using the ID32C ATB system (bioMerieux, France), chromogenic culture Chromagar Candida (R) (CHROMagar, France) and morphogenesis in corn meal agar. We studied 79 isolates, in which the most prevalent species using the system ID32C and PCR was C. albicans, followed by C. tropicalis, C. glabrata and C.krusei. PCR patterns obtained for the identification of clinical isolates were stable and consistent in the various independent studies and showed good reproducibility, concluding that PCR with species-specific primers that amplify genes ITS1 and ITS2 for rRNA or topoisomerase II primers is a very specific and sensitive method for the identification of C. glabrata, C. krusei, C. albicans, and with less specificity for C. tropicalis. (C) 2010 Revista Iberoamericana de Micologia. Published by Elsevier Espana, S.L. All rights reserved.
引用
收藏
页码:36 / 42
页数:7
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