Reduced graphene oxide nanosheets functionalized with poly(styrene sulfonate) as a peroxidase mimetic in a colorimetric assay for ascorbic acid

被引：84
作者：
Chen, Jing [1 ]
Ge, Jia [1 ]
Zhang, Lin [1 ]
Li, Zhaohui [1 ]
Li, Jianjun [1 ]
Sun, Yanjun [1 ]
Qu, Lingbo [1 ,2 ]
机构：
[1] Zhengzhou Univ, Coll Chem & Mol Engn, Zhengzhou 450001, Peoples R China
[2] Luoyang Inst Sci & Technol, Luoyang 471023, Peoples R China
来源：
MICROCHIMICA ACTA | 2016年 / 183卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Nanocomposites; Enzyme mimetic; Vitamin C; Orange juice; Photometry; Visual detection; GOLD NANOPARTICLES; CATALYTIC-ACTIVITY; VITAMIN-C; NANOCOMPOSITE; DOPAMINE; GLUCOSE; ENZYME; PROBES;
D O I：
10.1007/s00604-016-1826-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The authors describe a highly sensitive colorimetric method for the determination of ascorbic acid. It is based on the finding that nanosheets of reduced graphene oxide that were functionalized with poly(styrene sulfonate) (PSS-rGO) possess peroxidase-like activity that catalyzes the oxidization of the substrate 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to form a blue product (oxidized TMB; oxTMB) that can be quantified by photometry or visually. If ascorbic acid is present, a fraction of H2O2 will be consumed and hence less oxTMB (with an absorption peak at 652 nm) will be formed. Ascorbic acid can be quantified by photometry in the 0.8 to 60 mu M concentration range with a 0.15 mu M detection limit. The method was successfully applied to the determination of ascorbic acid in vitamin C tablets and orange juice.
引用
收藏
页码：1847 / 1853
页数：7
相关论文
共 29 条
[1]   MIL-53(Fe): A Metal-Organic Framework with Intrinsic Peroxidase-Like Catalytic Activity for Colorimetric Biosensing [J].
Ai, Lunhong ;
Li, Lili ;
Zhang, Caihong ;
Fu, Jian ;
Jiang, Jing .
CHEMISTRY-A EUROPEAN JOURNAL, 2013, 19 (45) :15105-15108
[2]   Oxidase-Like Activity of Polymer-Coated Cerium Oxide Nanoparticles [J].
Asati, Atul ;
Santra, Santimukul ;
Kaittanis, Charalambos ;
Nath, Sudip ;
Perez, J. Manuel .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (13) :2308-2312
[3]   In-situ synthesis and characterization of electrically conductive polypyrrole/graphene nanocomposites [J].
Bose, Saswata ;
Kuila, Tapas ;
Uddin, Md Elias ;
Kim, Nam Hoon ;
Lau, Alan K. T. ;
Lee, Joong Hee .
POLYMER, 2010, 51 (25) :5921-5928
[4]   Single Nanoparticle to 3D Supercage: Framing for an Artificial Enzyme System [J].
Cai, Ren ;
Yang, Dan ;
Peng, Shengjie ;
Chen, Xigao ;
Huang, Yun ;
Liu, Yuan ;
Hou, Weijia ;
Yang, Shengyuan ;
Liu, Zhenbao ;
Tan, Weihong .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (43) :13957-13963
[5]   Colorimetric determination of melamine based on the reversal of the mercury(II) induced inhibition of the light-triggered oxidase-like activity of gold nanoclusters [J].
Cao, Gen-Xia ;
Wu, Xiu-Ming ;
Dong, Yu-Ming ;
Li, Zai-Jun ;
Wang, Guang-Li .
MICROCHIMICA ACTA, 2016, 183 (01) :441-448
[6]   PSS-GN nanocomposites as highly-efficient peroxidase mimics and their applications in colorimetric detection of glucose in serum [J].
Chen, Jing ;
Ge, Jia ;
Zhang, Lin ;
Li, Zhaohui ;
Zhou, Saisai ;
Qu, Lingbo .
RSC ADVANCES, 2015, 5 (110) :90400-90407
[7]   Protein-directed in situ synthesis of platinum nanoparticles with superior peroxidase-like activity, and their use for photometric determination of hydrogen peroxide [J].
Chen, Lijian ;
Wang, Nan ;
Wang, Xindong ;
Ai, Shiyun .
MICROCHIMICA ACTA, 2013, 180 (15-16) :1517-1522
[8]   Intrinsic peroxidase-like activity of ferromagnetic nanoparticles [J].
Gao, Lizeng ;
Zhuang, Jie ;
Nie, Leng ;
Zhang, Jinbin ;
Zhang, Yu ;
Gu, Ning ;
Wang, Taihong ;
Feng, Jing ;
Yang, Dongling ;
Perrett, Sarah ;
Yan, Xiyun .
NATURE NANOTECHNOLOGY, 2007, 2 (09) :577-583
[9]   Determination of vitamin C in tropical fruits:: A comparative evaluation of methods [J].
Hernández, Y ;
Lobo, MG ;
González, M .
FOOD CHEMISTRY, 2006, 96 (04) :654-664
[10]   Graphene quantum dots as on-off-on fluorescent probes for chromium(VI) and ascorbic acid [J].
Huang, Shan ;
Qiu, Hangna ;
Zhu, Fawei ;
Lu, Shuangyan ;
Xiao, Qi .
MICROCHIMICA ACTA, 2015, 182 (9-10) :1723-1731
123