Clinical research assessment by flow cytometry of biomarkers for infectious stratification in an Emergency Department

被引：23

作者：

Bourgoin, Penelope ^{[1
,2
]}

Soliveres, Thomas ^{[3
]}

Ahriz, Dalia ^{[3
]}

Arnoux, Isabelle ^{[4
]}

Meisel, Christian ^{[5
,6
]}

Unterwalder, Nadine ^{[6
]}

Morange, Pierre-Emmanuel ^{[2
,4
]}

Michelet, Pierre ^{[3
]}

Malergue, Fabrice ^{[1
]}

Markarian, Thibaut ^{[3
]}

机构：

[1] Beckman Coulter Life Sci Immunotech, Dept Res & Dev, 130 Ave Lattre de Tassigny, F-13009 Marseille, France

[2] Aix Marseille Univ, C2VN Dept, INSERM, INRA, 27 Blvd Jean Moulin, F-13385 Marseille, France

[3] La Timone Hosp, Adult Emergency Dept, 264 Rue St Pierre, F-13385 Marseille, France

[4] La Timone Hosp, Hematol Lab Dept, 264 Rue St Pierre, F-13385 Marseille, France

[5] Charite Univ Med Berlin, Dept Med Immunol, Augustenburger Pl 1, D-13353 Berlin, Germany

[6] Lab Berlin Charite Vivantes GmbH, Dept Immunol, Sylter Str 2, D-13353 Berlin, Germany

来源：

BIOMARKERS IN MEDICINE | 2019年 / 13卷 / 16期

关键词：

bacterial infections; CD169; CD64; emergency department; flow cytometry; viral infections; C-REACTIVE PROTEIN; NEUTROPHIL CD64; BACTERIAL-INFECTION; DIAGNOSTIC MARKER; EXPRESSION; PROCALCITONIN; MONOCYTES; INDEX; TIME; CD35;

D O I：

10.2217/bmm-2019-0214

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aim: Management of patients with infections within the Emergency Department (ED) is challenging for practitioners, as the identification of infectious causes remains difficult with current techniques. A new combination of two biomarkers was tested with a new rapid flow cytometry technique. Materials & methods: Subjects from the ED were tested for their CD64 on neutrophils (nCD64) and CD169 on monocytes (mCD169) levels and results were compared to their clinical records. Results: Among 139 patients, 29% had confirmed bacterial infections and 5% viral infections. nCD64 and mCD169 respectively showed 88 and 86% sensitivity and 90 and 100% specificity for identifying subjects in bacterial or viral conditions. Conclusion: This point-of-care technique could allow better management of patients in the ED.

引用

页码：1373 / 1386

页数：14

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