Relationship of superoxide dismutase to rotator cuff injury/tear in a rat model

被引:5
作者
Uehara, Hirohisa [1 ,2 ]
Itoigawa, Yoshiaki [1 ]
Wada, Tomoki [1 ]
Morikawa, Daichi [1 ]
Koga, Akihisa [1 ]
Nojiri, Hidetoshi [2 ]
Kawasaki, Takayuki [2 ]
Maruyama, Yuichiro [1 ]
Ishijima, Muneaki [2 ]
机构
[1] Juntendo Univ, Dept Orthopaed Surg, Urayasu Hosp, 2-1-1 Tomioka, Chiba 2790021, Japan
[2] Juntendo Univ, Dept Med Orthopaed & Motor Organ, Grad Sch Med, Tokyo, Japan
关键词
oxidative stress; peroxiredoxin; rotator cuff degeneration; rotator cuff tear; superoxide dismutase; ANTIOXIDANT ENZYME PEROXIREDOXIN-5; OXIDATIVE-STRESS; SUPRASPINATUS TENDON; CELLS; DEGENERATION; PROGRESSION; ACTIVATION; APOPTOSIS; BIOLOGY;
D O I
10.1002/jor.25141
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Rotator cuff degeneration is one of the several factors that lead to rotator cuff tears. Oxidative stress and superoxide dismutase have been reported to be related to rotator cuff degeneration; however, the precise mechanism still remains unclear. In this study, we investigated the relationship of oxidative stress and superoxide dismutase to the degeneration of the rotator cuff using rat models. Eighty-four rats were used to create a collagenase-induced rotator cuff injury model (injury model) and a rotator cuff tear model (tear model). The controls were administered saline and had only a deltoid incision, respectively. We evaluated degeneration morphology of the rotator cuff using a degeneration score; dihydroethidium fluorescence intensity, which detects oxidative stress; gene expression; and superoxide dismutase activity. The rotator cuffs in the injury and tear models significantly increased degeneration scores and dihydroethidium fluorescence intensity. On the other hand, gene expression of superoxide dismutase isoform, superoxide dismutase 1, and superoxide dismutase activity were significantly decreased in the injury model but showed no significant difference in the tear model. These findings suggested that superoxide dismutase might not be associated with rotator cuff degeneration after tear but may be involved in degenerative rotator cuff without tear. However, we found that rotator cuff degeneration involves oxidative stress both with and without tear. Based on these findings, it is presumed that different treatments may be appropriate, depending on the state of rotator cuff degeneration, because the mechanisms of the degeneration may be different.
引用
收藏
页码:1006 / 1015
页数:10
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