Induction of protective immunity by DNA vaccination with Toxoplasma gondii HSP70, HSP30 and SAG1 genes

被引：34

作者：

Mohamed, RM ^{[1
]}

Aosai, F ^{[1
]}

Chen, M ^{[1
]}

Mun, HS ^{[1
]}

Norose, K ^{[1
]}

Belal, US ^{[1
]}

Piao, LX ^{[1
]}

Yano, A ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Med, Dept Infect & Host Def, Chuo Ku, Chiba 2608670, Japan

来源：

VACCINE | 2003年 / 21卷 / 21-22期

关键词：

Toxoplasma gondii; T.g.HSP70; gene gun vaccination;

D O I：

10.1016/s0264-410X(03)00157-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The vaccination with Toxoplasma gondii heat shock protein 70 (T.g.HSP70) gene (a virulent tachyzoite-specific) induced the most prominent reduction in T gondii loads in various organs of B6 and BALB/c mice at the acute and chronic phases of toxoplasmosis compared with T.g.HSP30 (a bradyzoite-specific) and SAG1(a tachyzoite-specific) genes. A single gene gun vaccination with 2 mug of T.g.HSP70 gene induced a significant reduction in the number of T gondii organisms compared with 50 mug of T.g.HSP70 gene vaccination by intramuscular (i.m.) or intraperitoneal (i.p.) injection. The vaccine effects of T.g.HSP70 gene persisted for more than 3 months. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：2852 / 2861

页数：10

共 55 条

[11] Receptor-mediated uptake of antigen/heat shock protein complexes results in major histocompatibility complex class I antigen presentation via two distinct processing pathways [J].

Castellino, F ;

Boucher, PE ;

Eichelberg, K ;

Mayhew, M ;

Rothman, JE ;

Houghton, AN ;

Germain, RN .

JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 191 (11) :1957-1964

[12] Anti-HSP70 autoantibody formation by B-l cells in Toxoplasma gondii-infected mice [J].

Chen, M ;

Aosai, F ;

Mun, HS ;

Norose, K ;

Hata, H ;

Yang, A .

INFECTION AND IMMUNITY, 2000, 68 (09) :4893-4899

[13] Gene gun intradermal DNA immunization followed by boosting with modified vaccinia virus Ankara:: enhanced CD8+T cell immunogenicity and protective efficacy in the influenza and malaria models [J].

Dégano, P ;

Schneider, J ;

Hannan, CM ;

Gilbert, SC ;

Hill, AVS .

VACCINE, 1999, 18 (7-8) :623-632

[14] Regulation and function of T-cell-mediated immunity during Toxoplasma gondii infection [J].

Denkers, EY ;

Gazzinelli, RT .

CLINICAL MICROBIOLOGY REVIEWS, 1998, 11 (04) :569-+

[15] Induction of protective immunity against toxoplasmosis in mice by DNA immunization with a plasmid encoding Toxoplasma gondii GRA4 gene [J].

Desolme, B ;

Mévélec, MN ;

Buzoni-Gatel, D ;

Bout, D .

VACCINE, 2000, 18 (23) :2512-2521

[16] Dendritic cells directly trigger NK cell functions:: Cross-talk relevant in innate anti-tumor immune responses in vivo [J].

Fernandez, NC ;

Lozier, A ;

Flament, C ;

Ricciardi-Castagnoli, P ;

Bellet, D ;

Suter, M ;

Perricaudet, M ;

Tursz, T ;

Maraskovsky, E ;

Zitvogel, L .

NATURE MEDICINE, 1999, 5 (04) :405-411

[17]

GUPTA RS, 1993, J MOL EVOL, V37, P573

[18] DNA vaccines: Immunology, application, and optimization [J].

Gurunathan, S ;

Klinman, DM ;

Seder, RA .

ANNUAL REVIEW OF IMMUNOLOGY, 2000, 18 :927-974

[19] Protective immunity against congenital toxoplasmosis with recombinant SAG1 protein in a guinea pig model [J].

Haumont, M ;

Delhaye, L ;

Garcia, L ;

Jurado, M ;

Mazzu, P ;

Daminet, V ;

Verlant, V ;

Bollen, A ;

Biemans, R ;

Jacquet, A .

INFECTION AND IMMUNITY, 2000, 68 (09) :4948-4953

[20] CpG motifs present in bacterial DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon gamma [J].

Klinman, DM ;

Yi, AK ;

Beaucage, SL ;

Conover, J ;

Krieg, AM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (07) :2879-2883

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