Induction of protective immunity by DNA vaccination with Toxoplasma gondii HSP70, HSP30 and SAG1 genes

被引：34

作者：

Mohamed, RM ^{[1
]}

Aosai, F ^{[1
]}

Chen, M ^{[1
]}

Mun, HS ^{[1
]}

Norose, K ^{[1
]}

Belal, US ^{[1
]}

Piao, LX ^{[1
]}

Yano, A ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Med, Dept Infect & Host Def, Chuo Ku, Chiba 2608670, Japan

来源：

VACCINE | 2003年 / 21卷 / 21-22期

关键词：

Toxoplasma gondii; T.g.HSP70; gene gun vaccination;

D O I：

10.1016/s0264-410X(03)00157-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The vaccination with Toxoplasma gondii heat shock protein 70 (T.g.HSP70) gene (a virulent tachyzoite-specific) induced the most prominent reduction in T gondii loads in various organs of B6 and BALB/c mice at the acute and chronic phases of toxoplasmosis compared with T.g.HSP30 (a bradyzoite-specific) and SAG1(a tachyzoite-specific) genes. A single gene gun vaccination with 2 mug of T.g.HSP70 gene induced a significant reduction in the number of T gondii organisms compared with 50 mug of T.g.HSP70 gene vaccination by intramuscular (i.m.) or intraperitoneal (i.p.) injection. The vaccine effects of T.g.HSP70 gene persisted for more than 3 months. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：2852 / 2861

页数：10

共 55 条

[1] Immunization with a DNA plasmid encoding the SAG1 (P30) protein of Toxoplasma gondii is immunogenic and protective in rodents [J].

Angus, CW ;

Klivington-Evans, D ;

Dubey, JP ;

Kovacs, JA .

JOURNAL OF INFECTIOUS DISEASES, 2000, 181 (01) :317-324

[2]

Aosai F, 2002, CELL STRESS CHAPERON, V7, P357, DOI 10.1379/1466-1268(2002)007<0357:TGDHSP>2.0.CO

[3]

[4] Protective immunity induced by vaccination with SAG1 gene-transfected cells against Toxoplasma gondii-infection in mice [J].

Aosai, F ;

Mun, HS ;

Norose, K ;

Chen, M ;

Hata, H ;

Kobayashi, M ;

Kiuchi, M ;

Stauss, HJ ;

Yano, A .

MICROBIOLOGY AND IMMUNOLOGY, 1999, 43 (01) :87-91

[5] Necrotic but not apoptotic cell death releases heat shock proteins, which deliver a partial maturation signal to dendritic cells and activate the NF-κB pathway [J].

Basu, S ;

Binder, RJ ;

Suto, R ;

Anderson, KM ;

Srivastava, PK .

INTERNATIONAL IMMUNOLOGY, 2000, 12 (11) :1539-1546

[6] Immune responses induced by gene gun or intramuscular injection of DNA vaccines that express immunogenic regions of the serine repeat antigen from Plasmodium falciparum [J].

Belperron, AA ;

Feltquate, D ;

Fox, BA ;

Horii, T ;

Bzik, DJ .

INFECTION AND IMMUNITY, 1999, 67 (10) :5163-5169

[7] Gene gun mediated vaccination is superior to manual delivery for immunisation with DNA vaccines expressing protective antigens from Yersinia pestis or Venezuelan Equine Encephalitis virus [J].

Bennett, AM ;

Phillpotts, RJ ;

Perkins, SD ;

Jacobs, SC ;

Williamson, ED .

VACCINE, 1999, 18 (7-8) :588-596

[8] Cutting edge:: Heat shock protein gp96 induces maturation and migration of CD11c+ cells in vivo [J].

Binder, RJ ;

Anderson, KM ;

Basu, S ;

Srivastava, PK .

JOURNAL OF IMMUNOLOGY, 2000, 165 (11) :6029-6035

[9] Saturation, competition, and specificity in interaction of heat shock proteins (hsp) gp96, hsp90, and hsp70 with CD11b+ cells [J].

Binder, RJ ;

Harris, ML ;

Ménoret, A ;

Srivastava, PK .

JOURNAL OF IMMUNOLOGY, 2000, 165 (05) :2582-2587

[10] Routes of plasmid DNA vaccination that prime murine humoral and cellular immune responses [J].

Böhm, W ;

Mertens, T ;

Schirmbeck, R ;

Reimann, J .

VACCINE, 1998, 16 (9-10) :949-954

← 1 2 3 4 5 6 →