Andrographolide Inhibits Biofilm and Virulence in Listeria monocytogenes as a Quorum-Sensing Inhibitor

被引:20
作者
Yu, Tao [1 ,2 ]
Jiang, Xiaojie [1 ]
Xu, Xiaobo [1 ]
Jiang, Congyi [3 ]
Kang, Rui [3 ]
Jiang, Xiaobing [3 ]
机构
[1] Xinxiang Univ, Sch Life Sci & Basic Med, Xinxiang 453000, Henan, Peoples R China
[2] Xinxiang Univ, Key Lab Biomed & Hlth Risk Warning Xinxiang City, Xinxiang 453000, Henan, Peoples R China
[3] Henan Normal Univ, Coll Life Sci, Henan Engn Lab Bioconvers Technol Funct Microbes, Xinxiang 453007, Henan, Peoples R China
来源
MOLECULES | 2022年 / 27卷 / 10期
关键词
Listeria monocytogenes; andrographolide; quorum sensing; biofilm; virulence; GENE-EXPRESSION; MOTILITY; LUXS; TEMPERATURE; ACTIVATION;
D O I
10.3390/molecules27103234
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Listeria monocytogenes is a major foodborne pathogen that can cause listeriosis in humans and animals. Andrographolide is known as a natural antibiotic and exhibits good antibacterial activity. We aimed to investigate the effect of andrographolide on two quorum-sensing (QS) systems, LuxS/AI-2 and Agr/AIP of L. monocytogenes, as well as QS-controlled phenotypes in this study. Our results showed that neither luxS expression nor AI-2 production was affected by andrographolide. Nevertheless, andrographolide significantly reduced the expression levels of the agr genes and the activity of the agr promoter P-2. Results from the crystal violet staining method, confocal laser scanning microscopy (CLSM), and field emission scanning electron microscopy (FE-SEM) demonstrated that andrographolide remarkably inhibited the biofilm-forming ability of L. monocytogenes 10403S. The preformed biofilms were eradicated when exposed to andrographolide, and reduced surviving cells were also observed in treated biofilms. L. monocytogenes treated with andrographolide exhibited decreased ability to secrete LLO and adhere to and invade Caco-2 cells. Therefore, andrographolide is a potential QS inhibitor by targeting the Agr QS system to reduce biofilm formation and virulence of L. monocytogenes.
引用
收藏
页数:13
相关论文
共 41 条
[1]   Identification of the agr locus of Listeria monocytogenes:: Role in bacterial virulence [J].
Autret, N ;
Raynaud, C ;
Dubail, I ;
Berche, P ;
Charbit, A .
INFECTION AND IMMUNITY, 2003, 71 (08) :4463-4471
[2]   Assessment of the roles of LuxS, S-ribosyl homocysteine, and autoinducer 2 in cell attachment during biofilm formation by Listeria monocytogenes EGD-e [J].
Belval, SC ;
Gal, L ;
Margiewes, S ;
Garmyn, D ;
Piveteau, P ;
Guzzo, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (04) :2644-2650
[3]   The arsenal of virulence factors deployed by Listeria monocytogenes to promote its cell infection cycle [J].
Camejo, Ana ;
Carvalho, Filipe ;
Reis, Olga ;
Leitao, Elsa ;
Sousa, Sandra ;
Cabanes, Didier .
VIRULENCE, 2011, 2 (05) :379-394
[4]   Andrographolide Inhibits ICAM-1 Expression and NF-κB Activation in TNF-α-Treated EA.hy926 Cells [J].
Chao, Che-Yi ;
Lii, Chong-Kuei ;
Tsai, I-Ting ;
Li, Chien-Chun ;
Liu, Kai-Li ;
Tsai, Chia-Wen ;
Chen, Haw-Wen .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2011, 59 (10) :5263-5271
[5]   Quorum Sensing Signal Synthesis May Represent a Selective Advantage Independent of Its Role in Regulation of Bioluminescence in Vibrio fischeri [J].
Chong, Grace ;
Kimyon, Oender ;
Manefield, Mike .
PLOS ONE, 2013, 8 (06)
[6]   The Effects of Chinese Herbal Medicines on the Quorum Sensing-Regulated Virulence in Pseudomonas aeruginosa PAO1 [J].
Chong, Yee Meng ;
How, Kah Yan ;
Yin, Wai Fong ;
Chan, Kok Gan .
MOLECULES, 2018, 23 (04)
[7]   Surveillance for Listeria monocytogenes and listeriosis, 1995-2004 [J].
Clark, C. G. ;
Farber, J. ;
Pagotto, F. ;
Ciampa, N. ;
Dore, K. ;
Nadon, C. ;
Bernard, K. ;
Ng, L. -K. .
EPIDEMIOLOGY AND INFECTION, 2010, 138 (04) :559-572
[8]   Assessing the Contributions of the LiaS Histidine Kinase to the Innate Resistance of Listeria monocytogenes to Nisin, Cephalosporins, and Disinfectants [J].
Collins, Barry ;
Guinane, Caitriona M. ;
Cotter, Paul D. ;
Hill, Colin ;
Ross, R. Paul .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2012, 78 (08) :2923-2929
[9]   Anaerobic expression of the gadE-mdtEF multidrug efflux operon is primarily regulated by the two-component system ArcBA through antagonizing the H-NS mediated repression [J].
Deng, Ziqing ;
Shan, Yue ;
Pan, Qing ;
Gao, Xiang ;
Yan, Aixin .
FRONTIERS IN MICROBIOLOGY, 2013, 4
[10]   Influence of temperature on biofilm formation by Listeria monocytogenes on various food-contact surfaces:: relationship with motility and cell surface hydrophobicity [J].
Di Bonaventura, G. ;
Piccolomini, R. ;
Paludi, D. ;
D'Orio, V. ;
Vergara, A. ;
Conter, M. ;
Ianieri, A. .
JOURNAL OF APPLIED MICROBIOLOGY, 2008, 104 (06) :1552-1561