Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)

被引：21

作者：

Kim, DU

Roh, TY

Lee, J

Noh, JY

Jang, YJ

Hoe, KL

Yoo, HS

Choi, MU ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Chem, Seoul 151742, South Korea

[2] Korea Res Inst Biosci & Biotechnol, Cell Cycle & Signal Transduct Res Unit, Taejon 305600, South Korea

[3] Seoul Natl Univ, Ctr Mol Catalysis, Seoul 151742, South Korea

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 1999年 / 1437卷 / 03期

关键词：

phospholipase D; cDNA sequence; expression; (Brassica oleracea var. capitata);

D O I：

10.1016/S1388-1981(99)00020-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned and expressed a full-length cDNA encoding a phospholipase D of type alpha (PLD alpha) from cabbage. Analysis of the cDNA predicted an 812-amino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDa and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：409 / 414

页数：6

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