The impact of the microbial load of genital mycoplasmas and gestational age on the intensity of intraamniotic inflammation

被引：50
作者：
Kacerovsky, Marian [1 ,6 ]
Pliskova, Lenka [2 ]
Bolehovska, Radka [2 ]
Skogstrand, Kristin [3 ,4 ]
Hougaard, David M. [3 ,4 ]
Tsiartas, Panagiotis [5 ]
Jacobsson, Bo [6 ,7 ]
机构：
[1] Charles Univ Prague, Dept Obstet & Gynecol, Prague, Czech Republic
[2] Charles Univ Prague, Inst Clin Biochem & Diagnost, Prague, Czech Republic
[3] Charles Univ Prague, Prague, Czech Republic
[4] Statens Serum Inst, Dept Clin Biochem & Immunol, Copenhagen, Denmark
[5] Papageorgiou Univ Hosp, Dept Obstet & Gynecol, Thessaloniki, Greece
[6] Sahlgrens Univ Hosp, Dept Obstet & Gynecol, Gothenburg, Sweden
[7] Inst Publ Hlth, Oslo, Norway
来源：
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 2012年 / 206卷 / 04期
关键词：
amniotic fluid; molecular microbiology; threshold cycle; ureaplasma; POLYMERASE-CHAIN-REACTION; UREAPLASMA-UREALYTICUM; AMNIOTIC-FLUID; CLINICAL-SIGNIFICANCE; CULTURE; HOMINIS; RUPTURE; ONSET; PPROM;
D O I：
10.1016/j.ajog.2012.01.004
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
OBJECTIVE: The objective of the study was to determine whether the bacterial load of genital mycoplasmas and gestation age are related to intraamniotic inflammatory response using the amniotic fluid levels of 18 inflammatory mediators. STUDY DESIGN: A prospective study of 145 women with singleton pregnancies complicated by preterm prelabor rupture of membranes between 240/ 7 and 366/ 7 weeks was conducted. Amniotic fluid was obtained from all women by transabdominal amniocentesis. The amounts of genital mycoplasma deoxyribonucleic acid were determined using the threshold cycle value and relative and absolute quantification techniques. A panel of multiple proteins was analyzed simultaneously using multiplex technology. RESULTS: Twenty-four women with the presence of genital mycoplasmas in amniotic fluid were included in the final analyses. The concentrations of 9 of the 18 evaluated proteins in the amniotic fluid correlated with bacterial load of genital mycoplasmas independent of the quantification technique used. CONCLUSION: The intensity of intraamniotic inflammatory response to genital mycoplasmas decreased with gestational age.
引用
收藏
页码：342.e1 / 342.e8
页数：8
相关论文
共 21 条
[1]   Polymerase chain reaction versus culture for detection of Ureaplasma urealyticum and Mycoplasma hominis in the urogenital tract of adults and the respiratory tract of newborns [J].
AbeleHorn, M ;
Wolff, C ;
Dressel, P ;
Zimmermann, A ;
Vahlensieck, W ;
Pfaff, F ;
Ruckdeschel, G .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1996, 15 (07) :595-598
[2]   Comparison of culture with the polymerase chain reaction for detection of Ureaplasma urealyticum in endotracheal aspirates of preterm infants [J].
Cunliffe, NA ;
Fergusson, S ;
Davidson, F ;
Ross, PW .
JOURNAL OF MEDICAL MICROBIOLOGY, 1996, 45 (01) :27-30
[3]   Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men [J].
Gdoura, Radhouane ;
Kchaou, Wiem ;
Chaari, Chiraz ;
Znazen, Abir ;
Keskes, Leila ;
Rebai, Tarek ;
Hammami, Adnane .
BMC INFECTIOUS DISEASES, 2007, 7 (1) :129
[4]   Expression of cytokines and chemokines in cervical and amniotic fluid:: Relationship to histological chorioamnionitis [J].
Holst, Rose-Marie ;
Laurini, Ricardo ;
Jacobsson, Bo ;
Samuelsson, Ellen ;
Savman, Karin ;
Doverhag, Christina ;
Wennerholm, Ulla-Britt ;
Hagberg, Henrik .
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE, 2007, 20 (12) :885-893
[5]   Microbial invasion and cytokine response in amniotic fluid in a Swedish population of women with preterm prelabor rupture of membranes [J].
Jacobsson, B ;
Mattsby-Baltzer, I ;
Andersch, B ;
Bokström, H ;
Holst, RM ;
Nikolaitchouk, N ;
Wennerholm, UB ;
Hagberg, H .
ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA, 2003, 82 (05) :423-431
[6]   Quantification of Ureaplasma urealyticum DNA in the amniotic fluid from patients in PTL and pPROM and its relation to inflammatory cytokine levels [J].
Jacobsson, Bo ;
Aaltonen, Riikka ;
Rantakokko-Jalava, Kaisu ;
Morken, Nils-Halvdan ;
Alanen, Anna .
ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA, 2009, 88 (01) :63-70
[7]   The microbial load with genital mycoplasmas correlates with the degree of histologic chorioamnionitis in preterm PROM [J].
Kacerovsky, Marian ;
Pliskova, Lenka ;
Bolehovska, Radka ;
Musilova, Ivana ;
Hornychova, Helena ;
Tambor, Vojtech ;
Jacobsson, Bo .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2011, 205 (03) :213.e1-213.e7
[8]   The bacterial load of Ureaplasma parvum in amniotic fluid is correlated with an increased intrauterine inflammatory response [J].
Kasper, David C. ;
Mechtler, Thomas P. ;
Reischer, Georg H. ;
Witt, Armin ;
Langgartner, Michaela ;
Pollak, Arnold ;
Herkner, Kurt R. ;
Berger, Angelika .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2010, 67 (02) :117-121
[9]   Biovar diversity of Ureaplasma urealyticum in amniotic fluid:: distribution, intrauterine inflammatory response and pregnancy outcomes [J].
Kim, M ;
Kim, G ;
Romero, R ;
Shim, SS ;
Kim, EC ;
Yoon, BH .
JOURNAL OF PERINATAL MEDICINE, 2003, 31 (02) :146-152
[10]   Diversity in cytokine response to bacteria associated with preterm birth by fetal membranes [J].
Menon, Ramkumar ;
Peltier, Morgan R. ;
Eckardt, Judith ;
Fortunato, Stephen J. .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2009, 201 (03) :306.e1-306.e6
123