Myeloperoxidase and adenosine-deaminase levels in the pleural fluid leakage induced by carrageenan in the mouse model of pleurisy

被引:0
作者
Fröde, TS
Medeiros, YS
机构
[1] Univ Fed Santa Catarina, Ctr Hlth Sci, Dept Clin Anal, BR-88040970 Florianopolis, SC, Brazil
[2] Univ Fed Santa Catarina, Ctr Biol Sci, Dept Pharmacol, BR-88040970 Florianopolis, SC, Brazil
关键词
adenosine-deaminase; myeloperoxidase; mouse pleurisy; inflammation;
D O I
暂无
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Background: Although myeloperoxidase (MPO) and adenosine-deaminase (ADA) levels are markers of activated leukocytes, both enzymes have not been currently addressed in inflammation models. Aims: This study evaluates whether the concentrations of these enzymes are significantly correlated with the content of leukocytes in a pleurisy model. Methods: The pleurisy was induced by carrageenan (1%) in mice, and the parameters analyzed 4 and 48 h after. Results: After the induction of inflammation (4 h), MPO and ADA levels peaked in parallel to neutrophils (p<0.01). Regarding the second phase of pleurisy (48 h), the highest concentrations of ADA were detected in parallel to the highest levels of mononuclears (p<0.01). At this time, MPO levels and neutrophils; remained elevated, although at lower levels than those found at 4 h. A significant positive correlation was found among neutrophils and MPO, and mononuclears; and ADA (p<0.01). Conclusions: These findings support the evidence that both enzymes are markers of the inflammatory process, and provide new tools for a better understanding of the immunoregulatory pathways that occur in inflammation.
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页码:223 / 227
页数:5
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