Differentiation of species of the Streptococcus bovis/equinus-complex by MALDI-TOF Mass Spectrometry in comparison to sodA sequence analyses

被引：47

作者：

Hinse, D. ^{[1
]}

Vollmer, T. ^{[1
]}

Erhard, M. ^{[2
]}

Welker, M. ^{[2
]}

Moore, E. R. B. ^{[3
,4
]}

Kleesiek, K. ^{[1
]}

Dreier, J. ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Univ Klin, Herz & Diabet Zentrum NRW, Inst Lab & Transfus Med, D-32545 Bad Oeynhausen, Germany

[2] AnagnosTec, Gesell Analyt Biochem & Diagnost mbH, Potsdam, Germany

[3] Univ Gothenburg, Sahlgrenska Acad, Dept Infect Dis, Gothenburg, Sweden

[4] Culture Collect Univ Gothenburg, Dept Clin Bacteriol, Sahlgrenska Univ Hosp, Gothenburg, Sweden

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2011年 / 34卷 / 01期

关键词：

Streptococcus bovis/equinus-complex; MALDI-TOF MS; Mass spectrometry; sodA sequence analysis; Microbial taxonomy; DESORPTION IONIZATION-TIME; DEPENDENT SUPEROXIDE-DISMUTASE; INFANTARIUS SUBSP COLI; 16S RIBOSOMAL-RNA; SP-NOV; GENUS STREPTOCOCCUS; IDENTIFICATION; BOVIS; ENDOCARDITIS; CLASSIFICATION;

D O I：

10.1016/j.syapm.2010.11.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Streptococcus bovis/equinus complex is a heterogeneous group within the group D streptococci with important clinical relevance regarding infective endocarditis, sepsis and colon carcinoma. The taxonomic identification of species and sub-species of this complex, by the standard methods remains difficult. In the present study, we compared the cluster analysis of 88 strains of species of the S. bovis/equinus complex by sequence analysis of the manganese-dependent superoxide dismutase gene (sodA) and by Matrix Assisted Laser Desorption/Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS). We observed a high congruence of strain grouping by MALDI-TOF MS in comparison with sodA sequence analyses, demonstrating the accuracy and reliability of MALDI-TOF MS in comparison to DNA sequence-based method. By generating mass spectra for each species and sub-species, we were able to discriminate all members of the S. bovis/equinus complex. Furthermore, we demonstrated reliable identifications to the species level by MALDI-TOF MS, independently of cultivation conditions. (C) 2010 Elsevier GmbH. All rights reserved.

引用

页码：52 / 57

页数：6

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