Inhibition of human ovarian tumor growth by cytokine-induced killer cells

被引：71
作者：
Kim, Hwan Mook
Kang, Jong Soon
Lim, Jaeseung
Park, Song-Kyu
Lee, Kiho
Yoon, Yeo Dae
Lee, Chang Woo
Lee, Ki Hoon
Han, Gyoonhee
Yang, Kyu-Hwan
Kim, Yeon Jin
Kim, Youngsoo
Han, Sang-Bae [1 ]
机构：
[1] Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, Chungbuk, South Korea
[2] Chungbuk Natl Univ, CBITRC, Cheongju 361763, Chungbuk, South Korea
[3] Korea Res Inst Biosci & Biotechnol KRIBB, Bioevaluat Ctr, Ochang 363883, Chungbuk, South Korea
[4] Innocell Ltd, Seoul 153769, South Korea
[5] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea
[6] Kyungwon Univ, Songnam 461701, Gyeonggi, South Korea
来源：
ARCHIVES OF PHARMACAL RESEARCH | 2007年 / 30卷 / 11期
关键词：
cytokine-induced killer cells; ovarian cancer; adoptive immunotherapy;
D O I：
10.1007/BF02977372
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Despite the recent improvement in the treatment of ovarian cancer, this disease is still leading cause of cancer death in women. In this study, the anti-tumor activity of cytokine-induced killer (CIK) cells against human ovarian cancer was evaluated in vitro and in vivo. Although CD3(+)CD56(+) cells were rare in fresh human peripheral blood mononuclear cells, they could expand more than 1,000-fold on day 14 in the presence of anti-CD3 antibody plus IL-2. At an effector-target cell ratio of 30:1, CIK cells destroyed 45% of SK-OV-3 human ovarian cancer cells, which was determined by the Cr-51-release assay. In addition, CIK cells at a dose of 23 million cells per mouse inhibited 73% of SK-OV-3 tumor growth in nude mouse xenograft assay. This study suggests that CIK cells may be used as an adoptive immunotherapy for patients with ovarian cancer.
引用
收藏
页码：1464 / 1470
页数：7
相关论文
共 30 条
[11]   Helper roles of NK and CD8+ T cells in the induction of tumor immunity -: Polarized dendritic cells as cancer vaccines [J].
Kalinski, Pawel ;
Nakamura, Yutaro ;
Watchmaker, Payal ;
Giermasz, Adam ;
Muthuswamy, Ravikumar ;
Mailliard, Robbie B. .
IMMUNOLOGIC RESEARCH, 2006, 36 (1-3) :137-146
[12]   Silencing human NKG2D, DAP10, and DAP12 reduces cytotoxicity of activated CD8+ T cells and NK cells [J].
Karimi, M ;
Cao, TM ;
Baker, JA ;
Verneris, MR ;
Soares, L ;
Negrin, RS .
JOURNAL OF IMMUNOLOGY, 2005, 175 (12) :7819-7828
[13]   Angelan isolated from Angelica gigas Nakai induces dendritic cell maturation through toll-like receptor 4 [J].
Kim, Jee Youn ;
Yoon, Yeo Dae ;
Ahn, Ji Mi ;
Kang, Jong Soon ;
Park, Song-Kyu ;
Lee, Kiho ;
Bin Song, Kyung ;
Kim, Hwan Mook ;
Han, Sang-Bae .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2007, 7 (01) :78-87
[14]  
KOMACKER M, 2006, INT J CANCER, V119, P1377
[15]  
Law KS, 2007, ANTICANCER RES, V27, P841
[16]   A phase I trial of autologous cytokine-induced killer cells for the treatment of relapsed Hodgkin disease and non-Hodgkin lymphoma [J].
Leemhuis, T ;
Wells, S ;
Scheffold, C ;
Edinger, M ;
Negrin, RS .
BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, 2005, 11 (03) :181-187
[17]  
LU PH, 1994, J IMMUNOL, V153, P1687
[18]   USE OF A SCID MOUSE HUMAN LYMPHOMA MODEL TO EVALUATE CYTOKINE-INDUCED KILLER-CELLS WITH POTENT ANTITUMOR CELL-ACTIVITY [J].
SCHMIDTWOLF, IGH ;
NEGRIN, RS ;
KIEM, HP ;
BLUME, KG ;
WEISSMAN, IL .
JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 174 (01) :139-149
[19]   5-aza-2′-deoxycytidine leads to down-regulation of aberrant p16INK4A RNA transcripts and restores the functional retinoblastoma protein pathway in hepatocellular carcinoma cell lines [J].
Suh, SI ;
Pyun, HY ;
Cho, JW ;
Baek, WK ;
Park, JB ;
Kwon, TK ;
Park, JW ;
Suh, MH ;
Carson, DA .
CANCER LETTERS, 2000, 160 (01) :81-88
[20]   Studies on inducing apoptosis effects and mechanism of CIK cells for MGC-803 gastric cancer cell lines [J].
Sun, S ;
Li, XM ;
Li, XD ;
Yang, WS .
CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS, 2005, 20 (02) :173-180
123