Inhibition of human ovarian tumor growth by cytokine-induced killer cells

被引：71

作者：

Kim, Hwan Mook

Kang, Jong Soon

Lim, Jaeseung

Park, Song-Kyu

Lee, Kiho

Yoon, Yeo Dae

Lee, Chang Woo

Lee, Ki Hoon

Han, Gyoonhee

Yang, Kyu-Hwan

Kim, Yeon Jin

Kim, Youngsoo

Han, Sang-Bae ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, Chungbuk, South Korea

[2] Chungbuk Natl Univ, CBITRC, Cheongju 361763, Chungbuk, South Korea

[3] Korea Res Inst Biosci & Biotechnol KRIBB, Bioevaluat Ctr, Ochang 363883, Chungbuk, South Korea

[4] Innocell Ltd, Seoul 153769, South Korea

[5] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea

[6] Kyungwon Univ, Songnam 461701, Gyeonggi, South Korea

来源：

ARCHIVES OF PHARMACAL RESEARCH | 2007年 / 30卷 / 11期

关键词：

cytokine-induced killer cells; ovarian cancer; adoptive immunotherapy;

D O I：

10.1007/BF02977372

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Despite the recent improvement in the treatment of ovarian cancer, this disease is still leading cause of cancer death in women. In this study, the anti-tumor activity of cytokine-induced killer (CIK) cells against human ovarian cancer was evaluated in vitro and in vivo. Although CD3(+)CD56(+) cells were rare in fresh human peripheral blood mononuclear cells, they could expand more than 1,000-fold on day 14 in the presence of anti-CD3 antibody plus IL-2. At an effector-target cell ratio of 30:1, CIK cells destroyed 45% of SK-OV-3 human ovarian cancer cells, which was determined by the Cr-51-release assay. In addition, CIK cells at a dose of 23 million cells per mouse inhibited 73% of SK-OV-3 tumor growth in nude mouse xenograft assay. This study suggests that CIK cells may be used as an adoptive immunotherapy for patients with ovarian cancer.

引用

页码：1464 / 1470

页数：7

共 30 条

[1] Expansion of cytotoxic CD3+CD56+ cells from peripheral blood progenitor cells of patients undergoing autologous hematopoietic cell transplantation [J].