Genomic analyses of ciprofloxacin-resistant Neisseria gonorrhoeae isolates recovered from the largest South American metropolitan area

被引：3
作者：
Cassu-Corsi, Dandara [1 ,6 ]
Santos, Fernanda F. [1 ]
Cayo, Rodrigo [1 ,2 ]
Martins, Willames M. B. S. [1 ]
Nodari, Carolina S. [1 ]
Almeida, Luiz G. P. [3 ]
Martins, Rafael A. [1 ]
Carvalho da Silva, Roberto J. [4 ]
Vasconcelos, Ana Tereza R. [3 ]
Pignatari, Antonio C. C. [1 ,5 ]
Gales, Ana C. [1 ,5 ]
机构：
[1] Univ Fed Sao Paulo UNIFESP, Dept Med, Lab ALERTA, Escola Paulista Med EPM, Sao Paulo, SP, Brazil
[2] Univ Fed Sao Paulo UNIFESP, Inst Ciencias Ambientais, Dept Ciencias Biol DCB, Lab Bacteriol & Imunol LIB,Setor Biol Mol Microbi, Diadema, SP, Brazil
[3] Lab Nacl Comp Cient LNCC, Petropolis, RJ, Brazil
[4] Ctr Referencia & Treinamento Doencas Sexualmente, Sao Paulo, SP, Brazil
[5] Univ Fed Sao Paulo UNIFESP, Dept Med, Lab Especial Microbiol Clin LEMC, Escola Paulista Med EPM,Disciplina Infectol, Sao Paulo, SP, Brazil
[6] Escola Paulista Med EPM, Dept Med, Programa Pos Grad Med Translac, Sao Paulo, SP, Brazil
来源：
GENOMICS | 2022年 / 114卷 / 04期
基金：
巴西圣保罗研究基金会;
关键词：
Gonococci; Gonorrhea; Macrolides; Fluoroquinolones; ST90/CC90; Phylogenetic analysis; GONOCOCCAL ANTIMICROBIAL RESISTANCE; GLOBAL QUALITY-ASSURANCE; EPIDEMIOLOGY; SUSCEPTIBILITY; IDENTIFICATION; SURVEILLANCE; CEFTRIAXONE; MECHANISMS; STRAINS;
D O I：
10.1016/j.ygeno.2022.110287
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We sequenced 13 Neisseria gonorrhoeae isolates exhibiting distinct susceptibility profiles and which were recovered over 12 years in the metropolitan region of Sao Paulo, Brazil. Whole Genome Sequencing (WGS) was performed on an Illumina MiSeq (TM) 2 x 300 bp paired-end reads. Bioinformatics analyses were carried out using CGE, PATRIC, and BLAST databases for manual curation of obtained genomes. Multilocus sequence typing (MLST) analysis identified seven STs, namely ST1580, ST1590, ST1901, ST1902, ST8161, ST9363, and ST15640. Moreover, a diversity of mutations was observed in MtrR/G45D-A39T, PIB/G120K-A121S, and PBP1/L421P. Mutations associated with sulfonamides (DHPS/R228S) and rifampicin (RNAP/H552N) were also detected, as well as tetracycline resistance determinants, namely rpsJ/V57M and tet(M). The results presented herein can contribute to the knowledge of N. gonorrhoeae strains circulating in Sao Paulo, Brazil.
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页数：6
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