T4SS-dependent TLR5 activation by Helicobacter pylori infection

被引:61
作者
Pachathundikandi, Suneesh Kumar [1 ]
Tegtmeyer, Nicole [1 ]
Arnold, Isabelle Catherine [2 ]
Lind, Judith [1 ]
Neddermann, Matthias [1 ]
Falkeis-Veits, Christina [3 ]
Chattopadhyay, Sujay [4 ]
Broenstrup, Mark [5 ]
Tegge, Werner [5 ]
Hong, Minsun [6 ]
Sticht, Heinrich [7 ]
Vieth, Michael [3 ]
Muller, Anne [2 ]
Backert, Steffen [1 ]
机构
[1] Friedrich Alexander Univ Erlangen Nuremberg, Div Microbiol, Dept Biol, Erlangen, Germany
[2] Univ Zurich, Inst Mol Canc Res, Zurich, Switzerland
[3] Klinikum Bayreuth, Inst Pathol, Bayreuth, Germany
[4] JIS Univ, JIS Inst Adv Studies & Res, Kolkata 700091, India
[5] Helmholtz Ctr Infect Res, Dept Chem Biol, Braunschweig, Germany
[6] Yonsei Univ, Div Biol Sci & Technol, Wonju, South Korea
[7] Friedrich Alexander Univ Erlangen Nuremberg, Div Bioinformat, Inst Biochem, Erlangen, Germany
关键词
GASTRIC EPITHELIAL-CELLS; IV SECRETION; PROTEIN; EXPRESSION; STIMULATION; RECOGNITION;
D O I
10.1038/s41467-019-13506-6
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Toll-like receptor TLR5 recognizes a conserved domain, termed D1, that is present in flagellins of several pathogenic bacteria but not in Helicobacter pylori. Highly virulent H. pylori strains possess a type IV secretion system (T4SS) for delivery of virulence factors into gastric epithelial cells. Here, we show that one of the H. pylori T4SS components, protein CagL, can act as a flagellin-independent TLR5 activator. CagL contains a D1-like motif that mediates adherence to TLR5(+) epithelial cells, TLR5 activation, and downstream signaling in vitro. TLR5 expression is associated with H. pylori infection and gastric lesions in human biopsies. Using TIr5-knockout and wild-type mice, we show that TLR5 is important for efficient control of H. pylori infection. Our results indicate that CagL, by activating TLR5, may modulate immune responses to H. pylori.
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页数:11
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