A "reactive" turn-on fluorescence probe for hypochlorous acid and its bioimaging application

被引：35

作者：

Gao, Yunling ^{[1
,3
]}

Pan, Yong ^{[1
]}

Chi, Yu ^{[1
]}

He, Yuanyuan ^{[2
]}

Chen, Haiyan ^{[2
]}

Nemykin, Victor N. ^{[3
]}

机构：

[1] Zhejiang Univ Technol, Coll Chem Engn, State Key Lab Breeding Base Green Chem Synth Tech, Hangzhou 310032, Zhejiang, Peoples R China

[2] China Pharmaceut Univ, Sch Engn, Dept Biomed Engn, Nanjing 210009, Peoples R China

[3] Univ Manitoba, Dept Chem, Winnipeg, MB R3T 2N2, Canada

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2019年 / 206卷

基金：

中国国家自然科学基金; 加拿大自然科学与工程研究理事会;

关键词：

aza-BODIPY; Aldoxime; Turn-on probe; Red-emission; AZA-BODIPY DYES; PHOTODYNAMIC THERAPY; LIVING CELLS; SOLAR-CELLS; TRIPLET PHOTOSENSITIZERS; HYDROGEN-PEROXIDE; MYELOPEROXIDASE; CHEMOSENSOR; DERIVATIVES; CHEMODOSIMETER;

D O I：

10.1016/j.saa.2018.07.090

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

An aza-BODIPY-CNOH probe attached aldoxime group demonstrated the specific detection for hypochlorous add by the turn-on red emission signal. NMR and HRMS experiments confirmed that the fluorescence originated from the oxidation degradation of the non-fluorescence, aldoxime-based aza-BODIPY-CNOH probe into the red-fluorescence, nitrile oxide-based aza-BODIPY compound aza-BODIPY-CNO. The aza-BODIPY-CNOH probe showed good biocompatibility and was low toxic to living cells as shown from MIT experiments. Living RAW264.7 cells imaging indicated the aza-BODIPY-CNOH probe had good permeability and either exogenous or endogenous HCIO caused the intracellular bright-red fluorescence, showing its potential hypochlorous acid-specific sensing ability in biological systems. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：190 / 196

页数：7

共 63 条

[1] HYPOCHLOROUS ACID DECOMPOSITION IN THE PH 5-8 REGION [J].