Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer

被引：4

作者：

Lin, Yu Kiat ^{[1
]}

Show, Pau Loke ^{[2
]}

Yap, Yee Jiun ^{[3
]}

Ariff, Arbakariya ^{[4
]}

Annuar, Mohamad Suffian Bin Mohamad ^{[1
]}

Lai, Oi Ming ^{[5
]}

Ling, Tau Chuan ^{[1
]}

Ng, Eng Poh ^{[6
]}

机构：

[1] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur, Malaysia

[2] Univ Nottingham Malaysia Campus, Fac Engn, Dept Chem & Environm Engn, Semenyih, Malaysia

[3] Univ Nottingham Malaysia Campus, Fac Engn, Dept Appl Math, Semenyih, Malaysia

[4] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Serdang, Malaysia

[5] Univ Putra Malaysia, Fac Biotechnol & Bimol Sci, Dept Bioproc Technol, Serdang, Malaysia

[6] Univ Sains Malaysia, Sch Chem Sci, Gelugor, Malaysia

来源：

FRONTIERS IN CHEMISTRY | 2018年 / 6卷

基金：

日本学术振兴会;

关键词：

liquid biphasic system; ethylene oxide-propylene oxide; extractive bioconversion; cyclodextrin; Bacillus cereus; AQUEOUS 2-PHASE SYSTEMS; ETHYLENE-OXIDE; PURIFICATION; TEMPERATURE;

D O I：

10.3389/fchem.2018.00448

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An extractive bioconversion conducted on soluble starch with cyclodextrin glycosyltransferase (CGTase) enzyme in ethylene oxide-propylene oxide (EOPO)/potassium phosphates liquid biphasic system (LBS) to extract gamma-cyclodextrin (g-CD) was examined. A range of EOPO (with potassium phosphates) molecular weights was screen to investigate the effect of the latter on the partioning efficency of CGTase and g-CD. The results show that the optimal top phase g-CD yield (74.4%) was reached in 35.0% (w/w) EOPO 970 and 10.0% (w/w) potassium phosphate with 2.0% (w/w) sodium chloride. A theoretical explanation for the effect of NaCl on g-CD was also presented. After a 2 h bioconversion process, a total of 0.87 mg/mL concentration of g-CD was produced in the EOPO/phosphates LBS top phase. After the extraction of top phase from LBS, four continuous repetitive batches were successfully conducted with relative CGTase activity of 1.00, 0.86, 0.45, and 0.40 respectively.

引用

页数：6

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