Construction of myoglobin imprinted polymer films by grafting from silicon surface

被引：30

作者：

Yildirim, Ertan ^{[1
]}

Turan, Eylem ^{[1
]}

Caykara, Tuncer ^{[1
]}

机构：

[1] Gazi Univ, Fac Sci, Dept Chem, TR-06500 Ankara, Turkey

来源：

JOURNAL OF MATERIALS CHEMISTRY | 2012年 / 22卷 / 02期

关键词：

TRANSFER RADICAL POLYMERIZATION; PROTEIN RECOGNITION; GOLD SURFACES; ANTIBODIES; SEPARATION;

D O I：

10.1039/c1jm12470f

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Molecularly imprinted polymer (MIP) films on silicon wafers were prepared by surface-initiated atom transfer polymerization (ATRP). 2-hydroxyethyl methacrylate and ethylene glycol dimethacrylate were used as the functional monomer and the cross-linking agent, respectively. Myoglobin (Mb) was selected as the template molecule to form the MIP films. Various characterization techniques including ellipsometry, contact-angle goniometer, atomic force microscopy, grazing angle-Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, were used to characterize the films. Ellipsometry was also used to quantify and compare the binding capacity of the MIP and nonimprinted polymer (NIP) films. The MIP films exhibited higher rebinding capacity than the NIP films at all solution concentrations of Mb. A selectivity coefficient of 3.15 was achieved for the MIP films prepared against the Mb template molecule.

引用

页码：636 / 642

页数：7

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