Maintenance of hemiround colonies and undifferentiated state of mouse induced pluripotent stem cells on carbon nanotube-coated dishes

被引：15
作者：
Akasaka, Tsukasa [1 ]
Yokoyama, Atsuro [1 ]
Matsuoka, Makoto [1 ]
Hashimoto, Takeshi [2 ]
Watari, Fumio [1 ]
机构：
[1] Hokkaido Univ, Grad Sch Dent Med, Kita Ku, Sapporo, Hokkaido 0608586, Japan
[2] Meijo Nano Carbon Co Ltd, Naka Ku, Nagoya, Aichi 4600002, Japan
来源：
CARBON | 2011年 / 49卷 / 07期
基金：
日本科学技术振兴机构;
关键词：
SELF-ASSEMBLED MONOLAYERS; CELLULAR BEHAVIOR; IN-VITRO; ADHESION; DIFFERENTIATION; SURFACES; GENERATION; MORPHOLOGY; INDUCTION; VIABILITY;
D O I：
10.1016/j.carbon.2011.01.061
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Induced pluripotent stem (iPS) cells have attracted worldwide interest. However, there have been only a few studies investigating effective culture substrates for feeder-free culturing for the maintenance of iPS cells. In this study, we cultured mouse iPS cells under feeder-free conditions on carbon nanotube (CNT)-coated dishes and then evaluated the colony morphology and differentiation state of the cells on the dishes. After 5 d of cultivation in a medium containing 15% fetal bovine serum (FBS) and leukemia inhibitory factor (LIF), the colonies on thick films of multi-walled CNTs (MWCNTs) were observed to be hemiround; further, the cells expressed early undifferentiation markers. On the other hand, the colonies on a cell culture polystyrene dish and a collagen-coated polystyrene dish showed indistinct outline and spread well, and most spreading cells only weakly expressed early undifferentiation markers. These results indicate that the thick films of MWCNTs could maintain hemiround colonies and undifferentiated state of mouse iPS cells cultured under feeder-free conditions. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2287 / 2299
页数：13
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