Cloning, mapping, and characterization of a human homologue of the yeast longevity assurance gene LAG1

被引：68

作者：

Pan, H

Qin, WX

Huo, KK

Wan, DF

Yu, Y

Xu, ZG

Hu, QD

Gu, KT

Zhou, XM

Jiang, HQ

Zhang, PP

Huang, Y

Li, YY ^{[1
]}

Gu, JR

机构：

[1] Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

[2] Inst Canc Res, Natl Lab Oncogenes & Related Genes, Shanghai 200032, Peoples R China

来源：

GENOMICS | 2001年 / 77卷 / 1-2期

关键词：

human LAG1 homologue; cDNA cloning; chromosomal mapping; two-hybrid; hepatocellular carcinoma; colony formation;

D O I：

10.1006/geno.2001.6614

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have identified LASS2, a previously unknown human homologue of the yeast longevity assurance gene LAG1. The LASS2 transcript is highly expressed in liver and kidney, which is very different from the expression of the previously identified human LAG1 homologue LAG1Hs-1. Radiation hybrid mapping studies indicated that LASS2 is located on chromosome 1q11. Yeast two-hybrid screening and glutathione S-transferase pull-down assays showed that the LASS2 protein interacts with several membrane-associated receptors or transporters. Furthermore, LASS2 protein was able to inhibit the colony formation of human hepatoma cells in vitro, which suggests that this gene may be involved in the regulation of cell growth.

引用

页码：58 / 64

页数：7

共 17 条

[1] Two endoplasmic reticulum (ER) membrane proteins that facilitate ER-to-Golgi transport of glycosylphosphatidylinositol-anchored proteins [J].