Degradation of the antibiotics norfloxacin and ciprofloxacin by a white-rot fungus and identification of degradation products

被引:265
作者
Prieto, Ailette [2 ,3 ,4 ]
Moeder, Monika [4 ]
Rodil, Rosario [5 ]
Adrian, Lorenz [1 ]
Marco-Urrea, Ernest [1 ]
机构
[1] UFZ Helmholtz Ctr Environm Res, Dept Isotope Biogeochem, D-04318 Leipzig, Germany
[2] Univ Basque Country, Dept Analyt Chem, Bilbao 48080, Spain
[3] Basque Fdn Sci, IKERBASQUE, Bilbao 48011, Spain
[4] UFZ Helmholtz Ctr Environm Res, Dept Analyt Chem, D-04318 Leipzig, Germany
[5] Univ Santiago de Compostela, IIAA Inst Food Anal & Res, Dept Analyt Chem Nutr & Food Sci, Santiago De Compostela 15782, Spain
基金
欧洲研究理事会;
关键词
Fluoroquinolones; Trametes versicolor; Pharmaceuticals; Biodegradation; Laccase; ENDOCRINE DISRUPTING CHEMICALS; WASTE-WATER; CHLORINATED HYDROXYBIPHENYLS; FLUOROQUINOLONE ENROFLOXACIN; TRAMETES-VERSICOLOR; LACCASE; ELIMINATION; SLUDGE; PHARMACEUTICALS; BIODEGRADATION;
D O I
10.1016/j.biortech.2011.08.055
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
More than 90% of the antibiotics ciprofloxacin (CIPRO) and norfloxacin (NOR) at 2 mg L-1 were degraded by Trametes versicolor after 7 days of incubation in malt extract liquid medium. In in vitro assays with purified laccase (16.7 nkat mL(-1)), an extracellular enzyme excreted constitutively by this fungus, 16% of CIPRO was removed after 20 h. The addition of the laccase mediator 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt led to 97.7% and 33.7% degradation of CIPRO and NOR, respectively. Inhibition of CIPRO and NOR degradation by the cytochrome P450 inhibitor 1-aminobenzotriazole suggests that the P450 system also plays a role in the degradation of the two antibiotics. Transformation products of CIPRO and NOR were monitored at different incubation times by triple-quadrupole and quadrupole time-of-flight mass spectrometry, and can be assigned to three different reaction pathways: (i) oxidation of the piperazinyl substituent, (ii) monohydroxylation, and (iii) formation of dimeric products. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:10987 / 10995
页数:9
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