Virucidal effect of disinfectants on spring viremia of carp virus

The virucidal effect of disinfectants against spring viremia of carp virus was assessed using EPC cells. Five kinds of disinfectants, i.e. benzalkonium chloride (BZC), alkyltoluene (AKT), chlorhexidine gluconate (CHG), cresol and ethanol, were examined at graduated concentrations of the disinfectants for different reaction periods, 30 s and 20 min. The minimum concentration of each disinfectant demonstrating >99.99% inactivation of the virus was 100 ppm (20 min) for BZC, 500 ppm (30 s) and 350 ppm (20 min) for AKT, 175 ppm (30 s) and 100 ppm (20 min) for CHG, 0.25% (30 s) and 200 ppm (20 min) for cresol and 50% (30 s) and 40% (20 min) for ethanol.