Magnetic enzyme reactors for isolation and study of heterogeneous glycoproteins

被引：23

作者：

Korecká, L

Jezová, J

Bílková, Z

Benes, M

Horák, D

Hradcová, O

Slováková, M

Viovy, JL

机构：

[1] Univ Pardubice, Dept Analyt Chem, Pardubice 53210, Czech Republic

[2] Univ Pardubice, Dept Biol & Biochem Sci, Pardubice 53210, Czech Republic

[3] Acad Sci Czech Republ, Inst Macromol Chem, Prague 16206, Czech Republic

[4] Inst Curie, CNRS, UMR 168, Lab Physicochim Curie, F-75231 Paris, France

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2005年 / 293卷 / 01期

关键词：

magnetic enzyme reactor (IMER); trypsin; chymotrypsin; papain; enzymes; neuraminidase; glycoproteins; magnetic separation;

D O I：

10.1016/j.jmmm.2005.02.030

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The newly developed magnetic micro- and nanoparticles with defined hydrophobicity and porosity were used for the preparation of magnetic enzyme reactors. Magnetic particles with immobilized proteolytic enzymes trypsin, chymotrypsin and papain and with enzyme neuraminidase were used to study the structure of heterogeneous glycoproteins. Factors such as the type of carrier, immobilization procedure, operational and storage stability, and experimental conditions were optimized. (c) 2005 Elsevier B.V. All rights reserved.

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页码：349 / 357

页数：9

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