Role of Hfq in Genome Evolution: Instability of G-Quadruplex Sequences in E. coli

被引:11
作者
Parekh, Virali J. [1 ]
Niccum, Brittany A. [2 ]
Shah, Rachna [3 ]
Rivera, Marisa A. [3 ]
Novak, Mark J. [4 ]
Geinguenaud, Frederic [5 ,6 ]
Wien, Frank [7 ]
Arluison, Veronique [8 ,9 ]
Sinden, Richard R. [1 ]
机构
[1] South Dakota Sch Mines & Technol, Dept Chem & Appl Biol Sci, Lab DNA Struct & Mutagenesis, Rapid City, SD 57701 USA
[2] Florida Inst Technol, Dept Math, Melbourne, FL 32901 USA
[3] Florida Inst Technol, Dept Biol Sci, Melbourne, FL 32901 USA
[4] South Dakota Sch Mines & Technol, Dept Chem & Appl Biol Sci, Rapid City, SD 57701 USA
[5] Univ Paris 13, Sorbonne Paris Cite, Plateforme CNanoMat, F-93017 Bobigny, France
[6] Univ Paris 13, Inserm U1148, Lab Vasc Translat Sci, UFR SMBH, F-93017 Bobigny, France
[7] Synchrotron SOLEIL, F-91192 Gif Sur Yvette, France
[8] Univ Paris Saclay, LLB, CEA Saclay, CEA,CNRS,UMR12, F-91191 Gif Sur Yvette, France
[9] Univ Paris, UFR Sci Vivant, 35 Rue Helene Brion, F-75205 Paris, France
基金
美国国家科学基金会;
关键词
genomic instability; quadruplex; DNA; mutagenesis; nucleoid; bacterial chromatin; STRESS-INDUCED MUTAGENESIS; I-MOTIF STRUCTURES; ESCHERICHIA-COLI; CIRCULAR-DICHROISM; FLUCTUATION ANALYSIS; GENE-EXPRESSION; LEADING-STRAND; DIRECT REPEATS; DNA-STRUCTURE; RNA;
D O I
10.3390/microorganisms8010028
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Certain G-rich DNA repeats can form quadruplex in bacterial chromatin that can present blocks to DNA replication and, if not properly resolved, may lead to mutations. To understand the participation of quadruplex DNA in genomic instability in Escherichia coli (E. coli), mutation rates were measured for quadruplex-forming DNA repeats, including (G(3)T)(4), (G(3)T)(8), and a RET oncogene sequence, cloned as the template or nontemplate strand. We evidence that these alternative structures strongly influence mutagenesis rates. Precisely, our results suggest that G-quadruplexes form in E. coli cells, especially during transcription when the G-rich strand can be displaced by R-loop formation. Structure formation may then facilitate replication misalignment, presumably associated with replication fork blockage, promoting genomic instability. Furthermore, our results also evidence that the nucleoid-associated protein Hfq is involved in the genetic instability associated with these sequences. Hfq binds and stabilizes G-quadruplex structure in vitro and likely in cells. Collectively, our results thus implicate quadruplexes structures and Hfq nucleoid protein in the potential for genetic change that may drive evolution or alterations of bacterial gene expression.
引用
收藏
页数:16
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