Activity of a flax pectin methylesterase promoter in transgenic tobacco pollen

被引：4

作者：

Lacoux, J ^{[1
]}

Duval, I ^{[1
]}

Dupré, P ^{[1
]}

Gutierrez, L ^{[1
]}

Lesueur, S ^{[1
]}

Roger, D ^{[1
]}

Lainé, E ^{[1
]}

机构：

[1] Univ Picardie, Fac Sci, Lab Biotechnol & Physiol Vegetales, F-80039 Amiens, France

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2003年 / 160卷 / 08期

关键词：

flax; Linum usitatissimum; Nicotiana tabacum; pectin methylesterase; pollen; promoter;

D O I：

10.1078/0176-1617-00786

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The regulatory region of the flax Lupme3 gene, which codes for a pectin methylesterase, contains two sequences (PB box) that are putative cis-active sequence elements thought to regulate transcription in pollen. The Lupme3 promoter was fused to the beta-glucuronidase (gus) reporter gene. The chimeric gene fusion was introduced into tobacco via Agrobacterium-mediated transformation. Expression of the reporter gene was monitored using a histochemical X-Gluc assay at different stages of pollen maturation and germination. The Lupme3 promoter was found to be active in germination-competent mature pollen and in pollen tube.

引用

页码：977 / 979

页数：3

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