Carrier-free Cultured Autologous Oral Mucosa Epithelial Cell Sheet (CAOMECS) for Corneal Epithelium Reconstruction: A Histological Study

被引:41
作者
Bardag-Gorce, Fawzia [1 ]
Oliva, Joan [1 ]
Wood, Andrew [4 ]
Hoft, Richard [3 ]
Pan, Derek [1 ]
Thropay, Jacquelyn [1 ]
Makalinao, Andrew [1 ]
French, Samuel W. [2 ]
Niihara, Yutaka [1 ,4 ]
机构
[1] Harbor UCLA Med Ctr, La BioMed, Dept Med, Torrance, CA 90502 USA
[2] Harbor UCLA Med Ctr, La BioMed, Dept Pathol, Torrance, CA 90502 USA
[3] Harbor UCLA Med Ctr, La BioMed, Div Ophthalmol, Torrance, CA 90502 USA
[4] Emmaus Life Sci Inc, Torrance, CA 90503 USA
关键词
cell sheet; corneal reconstruction; cultured autologous oral mucosa epithelial cell sheet (CAOMECS); epithelium; limbal stem cell deficiency; mucosal epithelial cells; oral mucosal epithelium; tissue engineering; OCULAR SURFACE RECONSTRUCTION; AMNIOTIC MEMBRANE; HUMAN LIMBAL; STEM-CELLS; EX-VIVO; TRANSPLANTATION; DEFICIENCY; INFLAMMATION; PTERYGIA; TISSUES;
D O I
10.1016/j.jtos.2014.12.003
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
This study investigates the therapeutic effects of carrier-free cultured autologous oral mucosa epithelial cell sheet (CAOMECS) transplantation for experimentally induced severe rabbit limbal stem cell deficiency (LSCD). Buccal biopsies were performed and CAOMECS were cultured and transplanted onto diseased corneas. Six-month follow-up examinations indicated that three out of four corneas with CAOMECS grafts showed a decrease in superficial vascularization, while almost all the sham corneas did not show a similar decrease. H&E staining of corneas showed that CAOMECS transplantation reduced blood vessel invasion of central cornea, reduced lymphocyte infiltration and fibrotic tissue formation. DeltaNp63 stained markedly in the grafted cornea and to a lesser extent in the sham corneas. PCNA and Ki-67 staining were much greater in the sham corneas than in the grafted and normal corneas. K3 and K13 staining demonstrated that CAOMECS transplanted corneas had much more K3- and less K13-positive cells compared to the sham corneas. Muc5AC was decreased in the central region of grafted corneas. Very little alpha-smooth muscle actin (aSMA) staining was detected in grafted corneas, while there was a greater amount of aSMA staining in sham corneas. Staining for anti-angiogenic factor TIMP -3 was also increased, and pro-angiogenic factor MMP-3 was decreased in grafted corneas compared to sham corneas. Our results indicate that CAOMECS grafts resulted in improved epithelialization of the corneal surface and decreased vascularization and fibrosis of the diseased corneas.
引用
收藏
页码:150 / 163
页数:14
相关论文
共 30 条
[1]   Microarray Analysis of Oral Mucosal Epithelial Cell Sheet [J].
Bardag-Gorce, Fawzia ;
Oliva, Joan ;
Wood, Andrew ;
Niihara, Hope ;
Makalinao, Andrew ;
Sabino, Sean ;
Pan, Derek ;
Thropay, Jacquelyn ;
Sota, Hiroyuki ;
Niihara, Yutaka .
TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2013, 10 (06) :362-370
[2]   Cultured Autologous Oral Mucosal Epithelial Cell Sheet (CAOMECS) Transplantation for the Treatment of Corneal Limbal Epithelial Stem Cell Deficiency [J].
Burillon, Carole ;
Huot, Laure ;
Justin, Virginie ;
Nataf, Serge ;
Chapuis, Francois ;
Decullier, Evelyne ;
Damour, Odile .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2012, 53 (03) :1325-1331
[3]   Cellular proliferation and leukocyte infiltration in the rabbit cornea after photorefractive keratectomy [J].
Gan, L ;
Hamberg-Nyström, H ;
Fagerholm, P ;
Van Setten, G .
ACTA OPHTHALMOLOGICA SCANDINAVICA, 2001, 79 (05) :488-492
[4]  
Garfias Y, 2009, MOL VIS, V15, P2068
[5]  
GIPSON IK, 1986, ARCH OPHTHALMOL-CHIC, V104, P1529
[6]   Comparison of prognostic value of Roper Hall and Dua classification systems in acute ocular burns [J].
Gupta, Noopur ;
Kalaivani, Mani ;
Tandon, Radhika .
BRITISH JOURNAL OF OPHTHALMOLOGY, 2011, 95 (02) :194-198
[7]   Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo on a temperature-responsive culture surface [J].
Hayashida, Y ;
Nishida, K ;
Yamato, M ;
Watanabe, K ;
Maeda, N ;
Watanabe, H ;
Kikuchi, A ;
Okano, T ;
Tano, Y .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2005, 46 (05) :1632-1639
[8]   Recent advances in cultivated epithelial transplantation [J].
Higa, Kazunari ;
Shimazaki, Jun .
CORNEA, 2008, 27 (08) :S41-S47
[9]   Transplantation of Cultivated Oral Mucosal Epithelium Prepared in Fibrin-Coated Culture Dishes [J].
Hirayama, Masatoshi ;
Satake, Yoshiyuki ;
Higa, Kazunari ;
Yamaguchi, Takefumi ;
Shimazaki, Jun .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2012, 53 (03) :1602-1609
[10]   Inhibition of angiogenesis induced by cerebral arteriovenous malformations using Gamma Knife irradiation [J].
Kilic, Kaya ;
Konya, Deniz ;
Kurtkaya, Ozlem ;
Sav, Aydin ;
Pamir, M. Necmettin ;
Kilic, Turker .
JOURNAL OF NEUROSURGERY, 2007, 106 (03) :463-469