Purification, crystallization and preliminary X-ray studies of two isoforms of Rubisco from Alcaligenes eutrophus

被引：3
作者：
Hansen, S [1 ]
Hough, E
Andersen, K
机构：
[1] Univ Tromso, Dept Chem, Protein Crystallog Grp, N-9037 Tromso, Norway
[2] Agr Univ Norway, IBF, Lab Microbial Gene Technol, N-1432 As, Norway
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1999年 / 55卷
关键词：
D O I：
10.1107/S0907444998010257
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Two different isoforms of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from Alcaligenes eutrophus have been purified and crystallized. Both isoforms crystallize in space group P4(3)2(1)2. Crystals of isoform I (unit-cell dimensions a = 112.0 and c = 402.7 Angstrom) diffract to 2.7 Angstrom, whereas isoform II (unit-cell dimensions a = 111.8 and c = 400.0 Angstrom) presently diffract to 3.2 Angstrom, using synchrotron radiation in both cases.
引用
收藏
页码：310 / 313
页数：4
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