Rapid and global detection and characterization of the constituents in ShengMai San by ultra-performance liquid chromatography-high-definition mass spectrometry

被引：50

作者：

Wu, Fangfang ^{[1
]}

Sun, Hui ^{[1
]}

Wei, Wenfeng ^{[1
]}

Han, Ying ^{[1
]}

Wang, Ping ^{[1
]}

Dong, Tianwei ^{[1
]}

Yan, Guangli ^{[1
]}

Wang, Xijun ^{[1
]}

机构：

[1] Heilongjiang Univ Chinese Med, Natl TCM Key Lab Serum Pharmacochem, Harbin 150040, Heilongjiang, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2011年 / 34卷 / 22期

关键词：

Constituent characterization; ShengMai San; Traditional Chinese medicine formula; UPLC-HDMS; TRADITIONAL CHINESE MEDICINE; CEREBRAL OXIDATIVE DAMAGE; OPHIOPOGON-JAPONICUS; SCHISANDRA-CHINENSIS; RAT PLASMA; MAI-SAN; LIGNAN CONSTITUENTS; HERBAL MEDICINE; IDENTIFICATION; SAPONINS;

D O I：

10.1002/jssc.201100253

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An ultra-performance liquid chromatographyhigh definition mass spectrometry (UPLC-HDMS) method was developed for detection and characterization of the chemical constituents in ShengMai San (SMS), a traditional Chinese medical formula (TCMF). The full-scan LC-MS/MS data sets combined with extra mass were acquired within 14?min using UPLC-HDMS in the MSE mode in a single injection. As a result, 92 compounds were identified by comparing the accurate mass and fragments information with that of the authentic standards as well as by MS analysis and the correlative references data. These constituents included ginsenosides, lignans, steroidal saponins and homoisoflavanones. Among them, 25-hydroxyginsenosides were discovered in SMS for the first time. Compare with the previous studies, our research detected more compounds and presented more rapid by applying UPLC-HDMS. It is concluded that a rapid and effective method has been established based on UPLC-HDMS with the utilization of MSE, which shows high sensitivity and resolution that is suitable for identifying the constituents of SMS, and this method could be applied to other TCMF.

引用

页码：3194 / 3199

页数：6

共 41 条

[1] Five new homoisoflavonoids from the tuber of Ophiopogon japonicus [J].