Calcyclin-binding protein inhibits proliferation, tumorigenicity, and invasion of gastric cancer

被引：64

作者：

Ning, Xiaoxuan ^{[1
,2
,3
]}

Sun, Shiren ^{[1
,2
,5
]}

Hong, Liu ^{[1
,2
]}

Liang, Jie ^{[1
,2
]}

Liu, Lili ^{[1
,2
]}

Han, Shuang ^{[1
,2
]}

Liu, Zhiguo ^{[1
,2
]}

Shi, Yongquan ^{[1
,2
]}

Li, Yuan

Gong, Weiqin ^{[3
]}

Zhang, Shanhong ^{[3
]}

Chen, Yu ^{[1
,2
]}

Guo, Xueyan ^{[1
,2
]}

Cheng, Yi ^{[4
]}

Wu, Kaichun ^{[1
,2
]}

Fan, Daiming ^{[1
,2
]}

机构：

[1] Fourth Mil Med Univ, Inst Digest Dis, Xijing Hosp, Xian 710032, Shaanxi Prov, Peoples R China

[2] Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi Prov, Peoples R China

[3] Fourth Mil Med Univ, Xijing Hosp, Dept Geriatr, Xian 710032, Shaanxi Prov, Peoples R China

[4] Wuhan Gen Hosp Guangzhou Command, Dept Gastroenterol, Wuhan, Hubei, Peoples R China

[5] Fourth Mil Med Univ, Dept Nephrol, Xijing Hosp, Xian 710032, Shaanxi Prov, Peoples R China

来源：

MOLECULAR CANCER RESEARCH | 2007年 / 5卷 / 12期

关键词：

D O I：

10.1158/1541-7786.MCR-06-0426

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP), a target protein of the S100 family, which includes S100A6, S100A1, S100A12, S100B, and S100P has been identified as a component of a novel ubiquitinylation complex leading to beta-catenin degradation. However, the function of CacyBP/SIP in gastric cancer has not been elucidated. In the present study, we prepared CacyBP/SIP overexpressing and knockdown cell lines of gastric cancer. Forced CacyBP/SIP expression inhibited the proliferation of gastric cancer cells, suppressed tumorigenicity in vitro, and prolonged the survival time of tumor-bearing nude mice. In addition, increased CacyBP/SIP repressed the invasive potential of gastric cancer cells. Conversely, the down-regulation of CacyBP/SIP by RNA interference showed the opposite effects. Further studies showed that depressed CacyBP/SIP increased the expression of total and nuclear beta-catenin at the protein level and elevated the transcriptional activity of Tcf/LEF. Taken together, our results suggest that CacyBP/SIP may be a potential inhibitor of cell growth and invasion in the gastric cancer cell, at least in part through the effect on beta-catenin protein expression and transcriptional activation of Tcf/LEF.

引用

页码：1254 / 1262

页数：9

共 36 条

[1] Calcyclin binding protein promotes DNA synthesis and differentiation in rat neonatal cardiomyocytes [J].