Immunoglobulin M antibodies against CRA and FRA recombinant antigens of Trypanosoma cruzi in chronic chagasic patients

被引：6
作者：
Vasconcelos, Romero H. T. [1 ]
Azevedo, Elisa A. N. [1 ]
Cavalcanti, Maria G. A. M. [2 ]
Silva, Edimilson D. [3 ,4 ]
Ferreira, Antonio G. P. [3 ,4 ]
Morais, Clarice N. L. [1 ]
Gomes, Yara M. [1 ,4 ]
机构：
[1] Ctr Pesquisas Aggeu Magalhaes Fiocruz, Dept Imunol, Recife, PE, Brazil
[2] Univ Fed Pernambuco, Hosp Univ Oswaldo Cruz, Recife, PE, Brazil
[3] Biomanguinhos Fiocruz, Dept Reat Diagnost, Rio De Janeiro, Brazil
[4] Programa Integrado Doenca Chagas Fiocruz, Rio De Janeiro, Brazil
来源：
HUMAN IMMUNOLOGY | 2011年 / 72卷 / 05期
关键词：
Immunoglobulin M; Recombinant antigens; Trypanosoma cruzi; Chagas disease; LINKED-IMMUNOSORBENT-ASSAY; IGG ANTIBODIES; CLINICAL FORMS; DISEASE; SERA; SERODIAGNOSIS; AMASTIGOTE;
D O I：
10.1016/j.humimm.2011.02.015
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Previous works of our research group have demonstrated aspects of the humoral immune response of chronic Chagas disease using the cytoplasmatic repetitive antigen (CRA) and the flagellar repetitive antigen (FRA) of Trypanosoma cruzi. The aim of this work was to analyze the presence of specific immunoglobulin M (IgM) antibodies in chronic chagasic patients using these recombinant antigens of T. cruzi. The positivity of IgM in chronic chagasic patients against CRA and FRA antigens was determined by indirect enzyme-linked immunosorbent assay. We reported no statistical significant differences between the levels of IgM for both recombinant antigens and the different chronic clinical forms of Chagas disease. However, a small proportion of chronic chagasic patients analyzed in this study was positive for this antibody isotype. The findings of this study indicate that the IgM antibodies cannot be used to elucidate the differences in the profile of humoral immune response among chronic chagasic patients with different clinical forms using the CRA and FRA recombinant antigens of T. cruzi. (C) 2011 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
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收藏
页码：402 / 405
页数：4
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