Human-derived feeder fibroblasts for the culture of epithelial cells for clinical use

被引：12
作者：
O'Callaghan, Anna R. [1 ]
Morgan, Louise [1 ]
Daniels, Julie T. [1 ]
Lewis, Mark P. [2 ]
机构：
[1] UCL, Inst Ophthalmol, Dept Ocular Biol & Therapeut, London EC1V 9EL, England
[2] Univ Loughborough, EPSRC MRC Ctr Doctoral Training Regenerat Med, Sch Sport Exercise & Hlth Sci, NCSEM, Loughborough LE11 3TU, Leics, England
来源：
REGENERATIVE MEDICINE | 2016年 / 11卷 / 06期
关键词：
cornea; limbal epithelial cells; limbal epithelial stem cell deficiency; limbal fibroblasts; oral mucosa; oral mucosal epithelial cells; oral mucosal fibroblasts; stem cell therapy; ORAL MUCOSAL EPITHELIUM; EX-VIVO EXPANSION; HUMAN LIMBAL; STEM-CELLS; AMNIOTIC MEMBRANE; CORNEAL; DIFFERENTIATION; EXPRESSION; THERAPY; SYSTEM;
D O I：
10.2217/rme-2016-0039
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Aim: To investigate human oral mucosal fibroblasts (HOMF) and human limbal fibroblasts (HLF) as alternatives to murine 3T3 feeder fibroblasts currently used to support epithelial cell expansion for the treatment of limbal epithelial stem cell deficiency. Methods: HLF and HOMF were compared with 3T3s for their ability to support the culture of human limbal epithelial cells and human oral mucosal epithelial cells. Results: HOMF, but not HLF, were equivalent to 3T3s in terms of the number of epithelial population doublings achieved. Human limbal epithelial cells co-cultured with HOMF or 3T3s had similar expression of corneal and putative stem cell markers. Conclusion: HOMF are a suitable and safer feeder fibroblast alternative to 3T3s for the production of epithelial cells for clinical use.
引用
收藏
页码：529 / 543
页数：15
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