A sensitive immunochromatographic assay using colloidal gold-antibody probe for rapid detection of fumonisin B1 in corn

被引：15

作者：

Wang, Xi-Chun ^{[1
,2
]}

Fan, Hai-Xin ^{[1
]}

Fan, Meng-Xue ^{[1
]}

Li, Fu-Hui ^{[1
]}

Feng, Shi-Bin ^{[1
]}

Li, Jin-Chun ^{[1
]}

Wu, Jin-Jie ^{[1
]}

Li, Yu ^{[1
]}

Wang, Jia-Sheng ^{[2
]}

机构：

[1] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei, Peoples R China

[2] Univ Georgia, Dept Environm Hlth Sci, Athens, GA 30602 USA

来源：

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT | 2016年 / 33卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Fumonisin B-1; monoclonal antibody; immunochromatographic assay; detection; corn; LINKED-IMMUNOSORBENT-ASSAY; STRIP TEST; IMMUNOASSAY; SAMPLES; B1; FOOD; OCHRATOXIN; PRODUCTS; FEEDS; CHROMATOGRAPHY;

D O I：

10.1080/19440049.2016.1213429

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A sensitive immunochromatographic assay (ICA) using a colloidal gold-antibody probe for the rapid detection of fumonisin B-1 (FB1) in corn samples was developed. The colour density of the test line correlated with the concentration of FB1 in the range 2-40 ngml(-1) by the assay, and the detection limit for FB1 was 2 ngml(-1). The linear range for FB1 was 50-1000 mu gkg(-1), and the visual limit detection of the test was 1000 mu gkg(-1) in corn samples. The ICA to detect FB1 is sensitive, specific and rapid. Specific anti-FB1 monoclonal antibody (mAb) and FB1-ovalbumin (FB1-OVA) conjugate antigen were prepared. FB1 mAb, labelled with colloidal gold, was used as the probe on the immunochromatographic strip. FB1-OVA and goat-anti-mouse IgG were coated onto a nitrocellulose (NC) membrane as test lines and control lines, respectively. FB1 in samples will competitively combines the FB1 mAb with the FB1-OVA in an NC membrane and the results are directly observed by the colour of the detection and quality control lines. The concentrations of FB1 mAb labelled with colloidal gold, detecting antigen and goat anti-mouse IgG, were optimised. The results indicate that the test strip is specific for FB1, with no cross-reactivity to other toxins. The strip assay for FB1 was simple, only needing one step without complicated assay performance and expensive equipment, and the total time for visual evaluation was less than 10min. A survey of 24 corn samples from Hefei, China, was performed with the test strip and HPLC, and the detection results showed that the developed ICA and the HPLC were in excellent agreement. Hence, the developed ICA can be used as a method for rapid detection of FB1 in corn samples.

引用

页码：1435 / 1443

页数：9

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