Compact surface plasmon resonance (SPR) immunosensor using multichannel for simultaneous detection of small molecule compounds

被引：47
作者：
Kawazumi, H [1 ]
Gobi, KV
Ogino, K
Maeda, H
Miura, N
机构：
[1] Kinki Univ, Dept Chem, Kyushu, Iizuka 8208555, Japan
[2] Kyushu Univ, Art Sci & Technol Ctr, Kasuga, Fukuoka 8168580, Japan
[3] Natl Inst Adv Ind Sci & Technol, Kyushu, Tosu 8410052, Japan
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2005年 / 108卷 / 1-2期
关键词：
surface plasmon resonance; biosensor; benzo[a]pyrene; antigen conjugate; competitive immunoreaction;
D O I：
10.1016/j.snb.2004.11.069
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A compact, portable SPR immunosensor designed for on site analysis of low-molecular-weight compounds by immunosensing is developed in the dimensions of 16 cm x 9 cm x 6 cm with four sensing microchannels. Highly sensitive and selective analysis of benzo[a]pyrene and 2-hydroxybiphenyl is realized by using an indirect competitive immunoreaction based on surface plasmon resonance (SPR) detection. A multi-microchannel flow-cell module employed here provides referencing channels to compensate bulk refractive index changes and temperature variations. It has been successfully used for simultaneous detection of the two compounds and for improvement of baseline stability by using the reference channels. Sensitivity of the compact, portable SPR sensor to ppb levels of the two compounds was equivalent to that obtained with a conventional SPR apparatus. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：791 / 796
页数：6
相关论文
共 16 条
[1]   Sub-ppb level detection of 2-hydroxybiphenyl using portable surface-plasmon-resonance immunosensor [J].
Gobi, KV ;
Kim, SJ ;
Harada, R ;
Miura, N .
ELECTROCHEMISTRY, 2003, 71 (06) :430-432
[2]   Highly sensitive detection of polycyclic aromatic hydrocarbons (PAHs) and association constants of the interaction between PAHs and antibodies using surface plasmon resonance immunosensor [J].
Gobi, KV ;
Sasaki, M ;
Shoyama, Y ;
Miura, N .
SENSORS AND ACTUATORS B-CHEMICAL, 2003, 89 (1-2) :137-143
[3]   Surface plasmon resonance sensors: review [J].
Homola, J ;
Yee, SS ;
Gauglitz, G .
SENSORS AND ACTUATORS B-CHEMICAL, 1999, 54 (1-2) :3-15
[4]   Imaging of electrochemical enzyme sensor on gold electrode using surface plasmon resonance [J].
Iwasaki, Y ;
Tobita, T ;
Kurihara, K ;
Horiuchi, T ;
Suzuki, K ;
Niwa, O .
BIOSENSORS & BIOELECTRONICS, 2002, 17 (09) :783-788
[5]  
LIDE DR, 2002, ANAL CHEM CONCENTRAT
[6]   Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method [J].
Masadome, T ;
Asano, Y ;
Imato, T ;
Ohkubo, S ;
Tobita, T ;
Tabei, H ;
Iwasaki, Y ;
Niwa, O ;
Fushinuki, Y .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2002, 373 (4-5) :222-226
[7]   A COMPACT SURFACE-PLASMON RESONANCE SENSOR FOR MEASUREMENT OF WATER IN PROCESS [J].
MATSUBARA, K ;
KAWATA, S ;
MINAMI, S .
APPLIED SPECTROSCOPY, 1988, 42 (08) :1375-1379
[8]   Surface plasmon resonance study of carbohydrate-carbohydrate interaction between various gangliosides and Gg3-carrying polystyrene [J].
Matsuura, K ;
Oda, R ;
Kitakouji, H ;
Kiso, M ;
Kitajima, K ;
Kobayashi, K .
BIOMACROMOLECULES, 2004, 5 (03) :937-941
[9]   Regenerable surface plasmon resonance (SPR)-based immunosensor for highly sensitive measurement of sub-ppb levels of benzo(a)pyrene [J].
Miura, N ;
Sasaki, M ;
Sakai, G ;
Gobi, KV .
CHEMISTRY LETTERS, 2002, (03) :342-343
[10]   PIEZOELECTRIC CRYSTAL IMMUNOSENSOR FOR SENSITIVE DETECTION OF METHAMPHETAMINE (STIMULANT DRUG) IN HUMAN URINE [J].
MIURA, N ;
HIGOBASHI, H ;
SAKAI, G ;
TAKEYASU, A ;
UDA, T ;
YAMAZOE, N .
SENSORS AND ACTUATORS B-CHEMICAL, 1993, 13 (1-3) :188-191
12