Speckle variance detection of microvasculature using swept-source optical coherence tomography

被引：574

作者：

Mariampillai, Adrian ^{[1
]}

Standish, Beau A. ^{[1
]}

Moriyama, Eduardo H. ^{[1
]}

Khurana, Mamta ^{[1
]}

Munce, Nigel R. ^{[1
]}

Leung, Michael K. K. ^{[1
]}

Jiang, James ^{[5
]}

Cable, Alex ^{[5
]}

Wilson, Brian C. ^{[1
]}

Vitkin, I. Alex ^{[1
,3
,4
]}

Yang, Victor X. D. ^{[1
,2
,6
]}

机构：

[1] Univ Toronto, Dept Med Biophys, Toronto, ON M5G 2M9, Canada

[2] Univ Toronto, Dept Neurosurg, Toronto, ON M5G 2M9, Canada

[3] Univ Toronto, Dept Radiat Oncol, Toronto, ON M5G 2M9, Canada

[4] Ontario Canc Inst, Toronto, ON M5G 2M9, Canada

[5] Thorlabs Inc, Newton, NJ 07860 USA

[6] Ryerson Univ, Dept Elect & Comp Engn, Toronto, ON M5B 2K3, Canada

来源：

OPTICS LETTERS | 2008年 / 33卷 / 13期

关键词：

D O I：

10.1364/OL.33.001530

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report on imaging of microcirculation. by calculating the speckle variance of optical coherence tomography (OCT) structural images acquired using a Fourier domain mode-locked swept-wavelength laser. The algorithm calculates interframe speckle variance in two-dimensional and three-dimensional OCT data sets and shows little dependence to the Doppler angle ranging from 75 degrees to 90 degrees. We demonstrate in vivo detection of blood flow in vessels as small as 25 mu m in diameter in a dorsal skinfold window chamber model with direct comparison with intravital fluorescence confocal microscopy. This technique can visualize vessel-size-dependent vascular shutdown and transient vascular occlusion during Visudyne photodynamic therapy and may provide opportunities for studying therapeutic effects of antivascular treatments without on exogenous contrast agent. (C) 2008 Optical Society of America.

引用

页码：1530 / 1532

页数：3

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