Endonuclease-based logic gates and sensors using magnetic force-amplified readout of DNA scission on cantilevers

被引:47
作者
Weizmann, Y [1 ]
Elnathan, R [1 ]
Lioubashevski, O [1 ]
Willner, I [1 ]
机构
[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel
关键词
D O I
10.1021/ja0533287
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The endonuclease scission of magnetic particles functionalized with sequence -specific DNAs, which are associated on cantilevers, is followed by the magnetic force-amplified readout of the reactions by the nano-mechanical deflection/retraction of the cantilevers. The systems are employed to develop AND or OR logic gates and to detect single base mismatch specificity of the endonucleases. The two endonucleases EcoRI (E-A) and Ascl (E-B) are used as inputs. The removal of magnetic particles linked to the cantilever by the duplexes 1/1a and 2/2a via the simultaneous cleavage of the DNAs by E-A and E-B leads to the retraction of the magnetically deflected cantilever and to the establishment of the "AND" gate, The removal of the magnetic particles linked to the cantilevers by the duplex 3/3a by either E-A or E-B leads to the retraction of the magnetically deflected cantilever and to the establishment of the "OR" gate. The magnetic force-amplified readout of endonuclease activities is also employed to reveal single base mismatch specificity of the biocatalysts.
引用
收藏
页码:12666 / 12672
页数:7
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