A new sensitive reversed-phase high-performance liquid chromatography method for the quantitative determination of metoclopramide in canine plasma

被引：10

作者：

Cossu, Massimo ^{[1
]}

Sanna, Vanna ^{[1
]}

Gavini, Elisabetta ^{[1
]}

Rassu, Giovanna ^{[1
]}

Giunchedi, Paolo ^{[1
]}

机构：

[1] Univ Sassari, Dipartimento Sci Farmaco, I-07100 Sassari, Italy

来源：

ANALYTICAL LETTERS | 2008年 / 41卷 / 05期

关键词：

metoclopramide hydrochloride; high-performance liquid chromatography (HPLC); pharmacokinetic study;

D O I：

10.1080/00032710801934601

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A specific and sensitive high-performance liquid chromatographic method was developed for the determination of metoclopramide in canine plasma. The procedure involves fast liquid-liquid extraction and analysis on an octadecyl silane (ODS) column. A preliminary pharmacokinetic study was performed by applying the developed method to a single oral administration of metoclopramide (MCP) to a dog. The validation method yielded good results regarding linearity, precision, accuracy, and specificity. The procedure is suitable for separation and quantification of MCP in canine plasma, enough to quantify 0.2ng/ml when 0.5ml of plasma is used. This assay procedure might be useful for the pharmacokinetic study of MCP in dogs.

引用

收藏

页码：767 / 778

页数：12

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