RAB6 and dynein drive post-Golgi apical transport to prevent neuronal progenitor delamination

被引：20

作者：

Brault, Jean-Baptiste ^{[1
]}

Bardin, Sabine ^{[1
]}

Lampic, Marusa ^{[1
]}

Carpentieri, Jacopo A. ^{[1
]}

Coquand, Laure ^{[1
,2
]}

Penisson, Maxime ^{[2
,3
,4
]}

Lachuer, Hugo ^{[1
]}

Victoria, Guiliana Soraya ^{[1
]}

Baloul, Sarah ^{[1
]}

El Marjou, Fatima ^{[1
]}

Boncompain, Gaelle ^{[1
]}

Miserey-Lenkei, Stephanie ^{[1
]}

Belvindrah, Richard ^{[2
,3
,4
]}

Fraisier, Vincent ^{[5
]}

Francis, Fiona ^{[2
,3
,4
]}

Perez, Franck ^{[1
]}

Goud, Bruno ^{[1
]}

Baffet, Alexandre D. ^{[1
,6
]}

机构：

[1] PSL Res Univ, Inst Curie, CNRS UMR144, Paris, France

[2] Sorbonne Univ, Paris, France

[3] INSERM, UMR S 1270, Paris, France

[4] Inst Fer Moulin, Paris, France

[5] Inst Curie, Cell & Tissue Imaging Facil PICT IBiSA, UMR 144, CNRS, Paris, France

[6] Inst Natl Sante & Rech Med INSERM, Paris, France

来源：

EMBO REPORTS | 2022年 / 23卷 / 10期

关键词：

cell polarity; dynein; neocortex development; polarized trafficking; RAB6; OUTER SUBVENTRICULAR ZONE; EPITHELIAL-CELL POLARITY; CYTOPLASMIC DYNEIN; NUCLEAR MIGRATION; RADIAL GLIA; DYNACTIN; RECRUITMENT; NEOCORTEX; MEMBRANE; GENE;

D O I：

10.15252/embr.202254605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Radial glial (RG) cells are the neural stem cells of the developing neocortex. Apical RG (aRG) cells can delaminate to generate basal RG (bRG) cells, a cell type associated with human brain expansion. Here, we report that aRG delamination is regulated by the post-Golgi secretory pathway. Using in situ subcellular live imaging, we show that post-Golgi transport of RAB6+ vesicles occurs toward the minus ends of microtubules and depends on dynein. We demonstrate that the apical determinant Crumbs3 (CRB3) is also transported by dynein. Double knockout of RAB6A/A' and RAB6B impairs apical localization of CRB3 and induces a retraction of aRG cell apical process, leading to delamination and ectopic division. These defects are phenocopied by knockout of the dynein activator LIS1. Overall, our results identify a RAB6-dynein-LIS1 complex for Golgi to apical surface transport in aRG cells, and highlights the role of this pathway in the maintenance of neuroepithelial integrity.

引用

页数：15