Rapid, synergistic extractive spectrophotometric determination of copper(II) by using sensitive chromogenic reagent N",N′"-bis[(E)-(4-fluorophenyl) methylidene]thiocarbonohydrazide

A rapid and simple spectrophotometric method was developed for the determination of copper(II) by using newly synthesized chromogenic reagent, N '',N '''-bis[(E)-(4-fluorophenyl)methylidene]thiocarbonohydrazide [bis(4-fluoroPM)TCH]. The reagent is highly sensitive and it forms yellow colored ternary complex with copper(II) in presence pyridine having composition 1:1:2 (M:L:Py) in the acidic pH range. Absorption of colored complex in amyl acetate is measured with reagent as a blank at lambda(max) 375 nm. The synergistic effect is observed due to pyridine forming adduct with reagent in the organic phase. Beer's law was obeyed in the concentration range from 2.0 to 14 mu g mL(-1) for copper(II)-[bis(4-fluoroPM)TCH]-Py complex. Molar absorptivity and Sandell's sensitivity values for Cu(II)-bis(4-fluoroPM)TCH]-Py complex are 0.42545 x 10(5) and 0.0014 mu g/cm(2), respectively. The selectivity of the developed method was checked in the presence of various foreign ions. The developed method showed relative standard deviation (R.S.D.) of 0.13% for n = 10. The composition of Cu(II)-[bis(4-fluoroPM)TCH]-Py complex was determined by known methods such as Job's method of continuous variation, mole ratio method and slope ratio method. It is found that the ternary complex is stable for more than 24 h. Various factors influencing on the degree of complexation, such as, effect of pH, reagent concentration, synergent concentration, solvent etc. were studied. The accuracy and reliability of method was verified by MS. This method is found to be simple, rapid and reproducible. (C) 2015 Published by Elsevier B.V.