Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions

被引：14
作者：
Madueo, L. [1 ]
Alvarez, H. M. [2 ]
Morelli, I. S. [1 ,3 ]
机构：
[1] Consejo Nacl Invest Cient & Tecn, Ctr Invest & Desarrollo Fermentac Ind, CINDEFI, UNLP,CCT La Plata, RA-1900 Buenos Aires, DF, Argentina
[2] Univ Nacl Patagonia San Juan Bosco, Fac Ciencias Nat, Ctr Reg Invest & Desarrollo Cient Tecnol CRIDECIT, Comodoro Rivadavia, Chubut, Argentina
[3] Comisin Investigaciones Cient Provincia Buenos Ai, Buenos Aires, DF, Argentina
来源：
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY | 2015年 / 12卷 / 07期
关键词：
Polycyclic aromatic hydrocarbons; Autochthonus bioaugmentation; Sphingobium; Patagonian soil; BACTERIAL COMMUNITY; OIL; BIOREMEDIATION; BIODEGRADATION; ENUMERATION; DYNAMICS; WATER;
D O I：
10.1007/s13762-014-0637-5
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The aim of this work was to investigate the effect of autochthonous bioaugmentation (ABA) in phenanthrene-contaminated Patagonian soil microcosms, maintained under arid conditions, on phenanthrene elimination and soil microbial community. The polycyclic aromatic hydrocarbon (PAH)-degrading strain Sphingobium sp. 22B previously isolated from the Patagonian soil and selected by its resistance to drying conditions was used as inoculant. The phenanthrene concentration, dehydrogenase activity and denaturing gradient electrophoresis of 16S rRNA gene were monitored during 230 days. The results showed that when the microcosms were maintained at 20 % of soil water-holding capacity (WHC), the phenanthrene biodegradation was drastically inhibited and changes in the genetic diversity of soil microbial community were not detected, and neither the ABA nor the biostimulation managed to overcome the inhibitory effects. When the moisture was slightly increased, reached 25 % WHC, the ABA showed a significant initial stimulatory effect on phenanthrene biodegradation, demonstrating the potential of ABA in PAH bioremediation process in semiarid Patagonia.
引用
收藏
页码：2317 / 2326
页数：10
相关论文
共 31 条
[1]   Effect of inoculation with putative plant growth-promoting rhizobacteria isolated from Pinus spp. on Pinus pinea growth, mycorrhization and rhizosphere microbial communities [J].
Barriuso, J. ;
Ramos Solano, B. ;
Santamaria, C. ;
Daza, A. ;
Gutierrez Manero, F. J. .
JOURNAL OF APPLIED MICROBIOLOGY, 2008, 105 (05) :1298-1309
[2]   Soil microbial activity and community composition: Impact of changes in matric and osmotic potential [J].
Chowdhury, Nasrin ;
Marschner, Petra ;
Burns, Richard G. .
SOIL BIOLOGY & BIOCHEMISTRY, 2011, 43 (06) :1229-1236
[3]   Sources and distribution of aliphatic and polyaromatic hydrocarbons in coastal sediments from the Ushuaia Bay (Tierra del Fuego, Patagonia, Argentina) [J].
Commendatore, Marta G. ;
Nievas, Marina L. ;
Amin, Oscar ;
Esteves, Jose L. .
MARINE ENVIRONMENTAL RESEARCH, 2012, 74 :20-31
[4]   Effects of the inoculant strain Sphingomonas paucimobilis 20006FA on soil bacterial community and biodegradation in phenanthrene-contaminated soil [J].
Coppotelli, B. M. ;
Ibarrolaza, A. ;
Del Panno, M. T. ;
Morelli, I. S. .
MICROBIAL ECOLOGY, 2008, 55 (02) :173-183
[5]   Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by Sphingomonas paucimobilis Strain 20006FA [J].
Coppotelli, Bibiana M. ;
Ibarrolaza, Agustin ;
Dias, Romina L. ;
Del Panno, Maria T. ;
Berthe-Corti, Luise ;
Morelli, Irma S. .
MICROBIAL ECOLOGY, 2010, 59 (02) :266-276
[6]   DGGE with genomic DNA: Suitable for detection of numerically important organisms but not for identification of the most abundant organisms [J].
de Araujo, Juliana Calabria ;
Schneider, Rene Peter .
WATER RESEARCH, 2008, 42 (20) :5002-5010
[7]   Effect of petrochemical sludge concentrations on microbial communities during soil bioremediation [J].
Del Panno, MT ;
Morelli, IS ;
Engelen, B ;
Berthe-Corti, L .
FEMS MICROBIOLOGY ECOLOGY, 2005, 53 (02) :305-316
[8]   Statistical analysis of denaturing gel electrophoresis (DGE) fingerprinting patterns [J].
Fromin, N ;
Hamelin, J ;
Tarnawski, S ;
Roesti, D ;
Jourdain-Miserez, K ;
Forestier, N ;
Teyssier-Cuvelle, S ;
Gillet, F ;
Aragno, M ;
Rossi, P .
ENVIRONMENTAL MICROBIOLOGY, 2002, 4 (11) :634-643
[9]   Effects of the inoculant strain Pseudomonas putida KT2442 (pNF142) and of naphthalene contamination on the soil bacterial community [J].
Gomes, NCM ;
Kosheleva, IA ;
Abraham, WR ;
Smalla, K .
FEMS MICROBIOLOGY ECOLOGY, 2005, 54 (01) :21-33
[10]   INDIGENOUS AND ENHANCED MINERALIZATION OF PYRENE, BENZO[A]PYRENE, AND CARBAZOLE IN SOILS [J].
GROSSER, RJ ;
WARSHAWSKY, D ;
VESTAL, JR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (12) :3462-3469
1234