Gammalinolenic acid, an unsaturated fatty acid with anti-inflammatory properties, blocks amplification of IL-1β production by human monocytes

Administration of gammalinolenic acid (GLA), an unsaturated fatty acid, reduces joint inflammation in patients with rheumatoid arthritis. Addition of GLA in vitro suppresses release of IL-1 beta from human monocytes stimulated with LPS. LPS-induced IL-lis release is followed by IL-1-induced IL-1 beta release, an amplification process termed autoinduction. We show here with peripheral blood monocytes from normal volunteers and from patients with rheumatoid arthritis by using IL-1 beta antagonist to block autoinduction and IL-la stimulation to simulate autoinduction that similar to 40% of IL-1 beta released from LPS-stimulated cells is attributable to autoinduction and that GLA reduces autoinduction of IL-1 beta while leaving the initial IL-1 beta response to LPS intact. Experiments with cells in which transcription and protein synthesis were blocked suggest that GLA induces a protein that reduces pro-IL-1 beta mRNA stability. IL-1 beta is important to host defense, but the amplification mechanism may be excessive in genetically predisposed patients. Thus, reduction of IL-1 beta autoinduction may be protective in some patients with endotoxic shock and with diseases characterized by chronic inflammation. The Journal of Immunology 2001.