A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans

被引:7
作者
Ahn, Jeong H. [1 ,2 ]
Rechsteiner, Andreas [3 ]
Strome, Susan [3 ]
Kelly, William G. [1 ]
机构
[1] Emory Univ, Dept Biol, Atlanta, GA 30322 USA
[2] Emory Univ, Program Genet & Mol Biol, Atlanta, GA 30322 USA
[3] Univ Calif Santa Cruz, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA
基金
美国国家卫生研究院;
关键词
ESS1 PROLYL ISOMERASE; GENOME-WIDE ANALYSIS; TERMINAL DOMAIN; C; ELEGANS; P-TEFB; IN-VIVO; MULTIDOMAIN CYCLOPHILIN; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; SR PROTEINS;
D O I
10.1371/journal.pgen.1006227
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The elongation phase of transcription by RNA Polymerase II (Pol II) involves numerous events that are tightly coordinated, including RNA processing, histone modification, and chromatin remodeling. RNA splicing factors are associated with elongating Pol II, and the interdependent coupling of splicing and elongation has been documented in several systems. Here we identify a conserved, multi-domain cyclophilin family member, SIG-7, as an essential factor for both normal transcription elongation and co-transcriptional splicing. In embryos depleted for SIG-7, RNA levels for over a thousand zygotically expressed genes are substantially reduced, Pol II becomes significantly reduced at the 3' end of genes, marks of transcription elongation are reduced, and unspliced mRNAs accumulate. Our findings suggest that SIG-7 plays a central role in both Pol II elongation and co-transcriptional splicing and may provide an important link for their coordination and regulation.
引用
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页数:27
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