Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction centre

被引：8

作者：

Bath, Petra ^{[1
]}

Banacore, Analia ^{[1
]}

Borjesson, Per ^{[1
]}

Bosman, Robert ^{[1
]}

Wickstrand, Cecilia ^{[1
]}

Safari, Cecilia ^{[1
]}

Dods, Robert ^{[1
]}

Ghosh, Swagatha ^{[1
]}

Dahl, Peter ^{[1
]}

Ortolani, Giorgia ^{[1
]}

Ulfarsdottir, Tinna Bjorg ^{[1
]}

Hammarin, Greger ^{[1
]}

Bonete, Maria Jose Garcia ^{[1
]}

Vallejos, Adams ^{[1
]}

Ostojic, Lucija ^{[1
]}

Edlund, Petra ^{[1
]}

Linse, Johanna Barbara ^{[1
]}

Andersson, Rebecka ^{[1
]}

Nango, Eriko ^{[2
,3
]}

Owada, Shigeki ^{[2
]}

Tanaka, Rie ^{[2
]}

Tono, Kensuke ^{[4
]}

Joti, Yasumasa ^{[4
]}

Nureki, Osamu ^{[5
]}

Luo, Fangjia ^{[6
]}

James, Daniel ^{[7
]}

Nass, Karol ^{[7
]}

Johnson, Philip J. M. ^{[7
]}

Knopp, Gregor ^{[7
]}

Ozerov, Dmitry ^{[7
]}

Cirelli, Claudio ^{[7
]}

Milne, Christopher ^{[7
]}

Iwata, So ^{[2
,6
]}

Branden, Gisela ^{[1
]}

Neutze, Richard ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Chem & Mol Biol, Lundbergslab Box 462, S-40530 Gothenburg, Sweden

[2] RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan

[3] Tohoku Univ, Inst Multidisciplinary Res Adv Mat, Aoba Ku, 2-1-1 Katahira, Sendai, Miyagi 9808577, Japan

[4] Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan

[5] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, 2-11-16 Yayoi, Tokyo 1130032, Japan

[6] Kyoto Univ, Grad Sch Med, Dept Cell Biol, Sakyo Ku, Yoshida Konoe Cho, Kyoto 6068501, Japan

[7] Paul Scherrer Inst, SwissFEL, Forschungsstr 111, CH-5232 Villigen, Switzerland

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2022年 / 78卷

基金：

欧洲研究理事会; 瑞典研究理事会; 欧盟地平线“2020”;

关键词：

membrane-protein crystallization; photosynthetic reaction centre; Blastochloris viridis; serial crystallography; lipidic cubic phase; microcrystals; REACTION-CENTER PROTEIN; RHODOPSEUDOMONAS-VIRIDIS; BLASTOCHLORIS-VIRIDIS; RHODOBACTER-SPHAEROIDES; MEMBRANE-PROTEINS; CRYSTALLIZATION; LIGHT; QUINONE; RESOLUTION; MECHANISM;

D O I：

10.1107/S2059798322004144

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Serial crystallography is a rapidly growing method that can yield structural insights from microcrystals that were previously considered to be too small to be useful in conventional X-ray crystallography. Here, conditions for growing microcrystals of the photosynthetic reaction centre of Blastochloris viridis within a lipidic cubic phase (LCP) crystallization matrix that employ a seeding protocol utilizing detergent-grown crystals with a different crystal packing are described. LCP microcrystals diffracted to 2.25 angstrom resolution when exposed to XFEL radiation, which is an improvement of 0.15 angstrom over previous microcrystal forms. Ubiquinone was incorporated into the LCP crystallization media and the resulting electron density within the mobile Q(B) pocket is comparable to that of other cofactors within the structure. As such, LCP microcrystallization conditions will facilitate time-resolved diffraction studies of electron-transfer reactions to the mobile quinone, potentially allowing the observation of structural changes associated with the two electron-transfer reactions leading to complete reduction of the ubiquinone ligand.

引用

页码：698 / 708

页数：11

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