Mechanisms of fibronectin-binding protein A (FnBPA110-263) vaccine efficacy in Staphylococcus aureus sepsis versus skin infection

被引：7
作者：
Zhang, Rui [2 ]
Li, Sun [1 ]
Zhang, Xiao-Kai [1 ]
Wang, Yu [1 ]
Yang, Liu-Yang [1 ]
Zeng, Hao [1 ]
Yan, Da-Peng [1 ]
Zou, Quan-Ming [1 ]
Zuo, Qian-Fei [1 ]
机构：
[1] Army Med Univ, Coll Pharm, Natl Engn Res Ctr Immunol Prod, Dept Microbiol & Biochem Pharm, Chongqing 400038, Peoples R China
[2] ChengDu Mil Gen Hosp, Clin Lab, Chengdu 610083, Sichuan, Peoples R China
来源：
CLINICAL IMMUNOLOGY | 2018年 / 194卷
基金：
中国国家自然科学基金;
关键词：
Staphylococcus aureus; Fibronectin-binding protein A; Vaccine; Th17; Immune protection; VANCOMYCIN-RESISTANT ENTEROCOCCUS; HYPER-IGE SYNDROME; PROTECTION; INTERLEUKIN-17A; RESPONSES; DOMAIN; MICE;
D O I：
10.1016/j.clim.2018.05.007
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Increasing rates of life-threatening infections and decreasing susceptibility to antibiotics urge an effective vaccine targeting Staphylococcus aureus. Here we investigate the role of cellular immunity in FnBPA(110-263) mediated protection in Staphylococcus aureus infection. This study revealed FnBPA(110-263) broadly protected mice from seven FnBPA isotypes strains in the sepsis model. FnBPA(110-263) immunized B-cell deficient mice were protected against lethal challenge, while T-cell deficient mice were not. Reconstituting mice with FnBPA(110-263) specific CD4 + T-cells conferred antigen specific protection. In vitro assays indicated that isolated FnBPA(110-263) specific splenocytes from immunized mice produced abundant IL-17A. IL-17A deficient mice were not protected from a lethal challenge by FnBPA(110-263) vaccination. Moreover, neutralizing IL-17A, but not IFN-gamma,reverses FnBPA(110-263)-induced protective efficacy in sepsis and skin infection model. These findings suggest that IL-17A producing Th17 cells play an essential role in FnBPA(110-263) vaccine-mediated defense against S. aureus sepsis and skin infection in mice.
引用
收藏
页码：1 / 8
页数：8
相关论文
共 20 条
  • [1] Ahmed MH, 2014, NAT PROD COMMUN, V9, P221
  • [2] [Anonymous], HUM VACCIN IMMUNOTHE, DOI DOI 10.4161/HV.18946
  • [3] Vaccine composition formulated with a novel TLR7-dependent adjuvant induces high and broad protection against Staphylococcus aureus
    Bagnoli, Fabio
    Fontana, Maria Rita
    Soldaini, Elisabetta
    Mishra, Ravi P. N.
    Fiaschi, Luigi
    Cartocci, Elena
    Nardi-Dei, Vincenzo
    Ruggiero, Paolo
    Nosari, Sarah
    De Falco, Maria Grazia
    Lofano, Giuseppe
    Marchi, Sara
    Galletti, Bruno
    Mariotti, Paolo
    Bacconi, Marta
    Torre, Antonina
    Maccari, Silvia
    Scarselli, Maria
    Rinaudo, C. Daniela
    Inoshima, Naoko
    Savino, Silvana
    Mori, Elena
    Rossi-Paccani, Silvia
    Baudner, Barbara
    Pallaoro, Michele
    Swennen, Erwin
    Petracca, Roberto
    Brettoni, Cecilia
    Liberatori, Sabrina
    Norais, Nathalie
    Monaci, Elisabetta
    Wardenburg, Juliane Bubeck
    Schneewind, Olaf
    O'Hagan, Derek T.
    Valiante, Nicholas M.
    Bensi, Giuliano
    Bertholet, Sylvie
    De Gregorio, Ennio
    Rappuoli, Rino
    Grandi, Guido
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (12) : 3680 - 3685
  • [4] Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection
    Brown, Aisling F.
    Murphy, Alison G.
    Lalor, Stephen J.
    Leech, John M.
    O'Keeffe, Kate M.
    Mac Aogain, Micheal
    O'Halloran, Dara P.
    Lacey, Keenan A.
    Tavakol, Mehri
    Hearnden, Claire H.
    Fitzgerald-Hughes, Deirdre
    Humphreys, Hilary
    Fennell, Jerome P.
    van Wamel, Willem J.
    Foster, Timothy J.
    Geoghegan, Joan A.
    Lavelle, Ed C.
    Rogers, Thomas R.
    McLoughlin, Rachel M.
    [J]. PLOS PATHOGENS, 2015, 11 (11)
  • [5] IL-17 is essential for host defense against cutaneous Staphylococcus aureus infection in mice
    Cho, John S.
    Pietras, Eric M.
    Garcia, Nairy C.
    Ramos, Romela Irene
    Farzam, David M.
    Monroe, Holly R.
    Magorien, Julie E.
    Blauvelt, Andrew
    Kolls, Jay K.
    Cheung, Ambrose L.
    Cheng, Genhong
    Modlin, Robert L.
    Miller, Lloyd S.
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2010, 120 (05) : 1762 - 1773
  • [6] Community-associated meticillin-resistant Staphylococcus aureus
    DeLeo, Frank R.
    Otto, Michael
    Kreiswirth, Barry N.
    Chambers, Henry F.
    [J]. LANCET, 2010, 375 (9725) : 1557 - 1568
  • [7] The remarkably multifunctional fibronectin binding proteins of Staphylococcus aureus
    Foster, T. J.
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2016, 35 (12) : 1923 - 1931
  • [8] Effect of an Investigational Vaccine for Preventing Staphylococcus aureus Infections After Cardiothoracic Surgery A Randomized Trial
    Fowler, Vance G., Jr.
    Allen, Keith B.
    Moreira, Edson D., Jr.
    Moustafa, Moustafa
    Isgro, Frank
    Boucher, Helen W.
    Corey, G. Ralph
    Carmeli, Yehuda
    Betts, Robert
    Hartzel, Jonathan S.
    Chan, Ivan S. F.
    McNeely, Tessie B.
    Kartsonis, Nicholas A.
    Guris, Dalya
    Onorato, Matthew T.
    Smugar, Steven S.
    DiNubile, Mark J.
    Sobanjo-ter Meulen, Ajoke
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2013, 309 (13): : 1368 - 1378
  • [9] Differential Roles of Interleukin-17A and-17F in Host Defense against Mucoepithelial Bacterial Infection and Allergic Responses
    Ishigame, Harumichi
    Kakuta, Shigeru
    Nagai, Takeshi
    Kadoki, Motohiko
    Nambu, Aya
    Komiyama, Yutaka
    Fujikado, Noriyuki
    Tanahashi, Yuko
    Akitsu, Aoi
    Kotaki, Hayato
    Sudo, Katsuko
    Nakae, Susumu
    Sasakawa, Chihiro
    Iwakura, Yoichiro
    [J]. IMMUNITY, 2009, 30 (01) : 108 - 119
  • [10] Lethal CD4 T Cell Responses Induced by Vaccination Against Staphylococcus aureus Bacteremia
    Karauzum, Hatice
    Haudenschild, Christian C.
    Moore, Ian N.
    Mahmoudieh, Mahta
    Barber, Daniel L.
    Datta, Sandip K.
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2017, 215 (08) : 1231 - 1239
12